Revision as of 09:13, 24 September 2008

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Things we did today

Grace

Margaret

1st lane is ladder, 2nd lane is rep amplified from a colony, 3rd lane is rep amplified from a restriction product of rep from the same colony.

Norman

x6 parallel MCS replacements fragment by PCR amplification. PCR out each MCS replacement insert with HindIII-VF2BB_fx._sb.1 and BamHI-VRBB_rx._sb.1
1. BBa_I52002 from pSB4A5 (Spring 2008 Plate 1022 1C)
2. BBa_I52001 from pSB4T5 (Spring 2008 Plate 1020 1A)
3. BBa_I52002 from BBa_I51020 (BBa_I51020 Base Vector from glycerol stock Inventory INV-Z80-R1-A3)
4. BBa_P1010 from pSB1A3 (pSB1A3 from glycerol stock Inventory INV-Z80-R1-A3)
5. BBa_P1010 from pSB1A7 (pSB1A7 from glycerol stock Inventory INV-Z80-R1-A3)
6. BBa_J33207 from pSB1A2 (BBa_J33207 harboring pSB1A2 from plasmid prep)
x2 Controls
1. positive control: BBa_K125805 from pSB1A3 (BBa_K125805 harboring pSB1A3 from glycerol stock Inventory INV-Z80-R1-D4)
2. negative control: H₂O

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

@@ Line 17: / Line 17: @@
 :*PCR of rep, ran a gel to verify size, and exo-sap. will send in tomorrow
+===PCR: pRL1383a MCS Replacement===
+:'''Norman'''
+* x6 parallel MCS replacements fragment by PCR amplification.  PCR out each MCS replacement insert with HindIII-VF2BB_fx._sb.1 and BamHI-VRBB_rx._sb.1
+*# BBa_I52002 from pSB4A5 (Spring 2008 Plate 1022 1C)
+*# BBa_I52001 from pSB4T5 (Spring 2008 Plate 1020 1A)
+*# BBa_I52002 from BBa_I51020 (BBa_I51020 Base Vector from glycerol stock Inventory [http://packrat.stjohn.hawaii.edu/prestinglab/wiki/INV-Z80-R1-A3 INV-Z80-R1-A3])
+*# BBa_P1010 from pSB1A3 (pSB1A3 from glycerol stock Inventory [http://packrat.stjohn.hawaii.edu/prestinglab/wiki/INV-Z80-R1-A3 INV-Z80-R1-A3])
+*# BBa_P1010 from pSB1A7 (pSB1A7 from glycerol stock Inventory [http://packrat.stjohn.hawaii.edu/prestinglab/wiki/INV-Z80-R1-A3 INV-Z80-R1-A3])
+*# BBa_J33207 from pSB1A2 ([http://partsregistry.org/wiki/index.php/Part:BBa_J33207 BBa_J33207] harboring pSB1A2 from plasmid prep)
+* x2 Controls
+*# positive control: [http://partsregistry.org/wiki/index.php/Part:BBa_K125805 BBa_K125805] from pSB1A3 ([http://partsregistry.org/wiki/index.php/Part:BBa_K125805 BBa_K125805] harboring pSB1A3 from glycerol stock Inventory [http://packrat.stjohn.hawaii.edu/prestinglab/wiki/INV-Z80-R1-D4 INV-Z80-R1-D4])
+*# negative control: H<sub>2</sub>O