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Team:Hawaii/Notebook/2008-10-16
From 2008.igem.org
(Difference between revisions)
(→Verification of transformants) |
(margaret) |
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| Line 21: | Line 21: | ||
:* Colony PCR of transformants | :* Colony PCR of transformants | ||
::* None of the colonies tested positive for their desired inserts | ::* None of the colonies tested positive for their desired inserts | ||
| + | |||
| + | ===Transformation=== | ||
| + | :<strong> Margaret</strong> | ||
| + | {|class=wikitable border=1 align=center | ||
| + | !Construct | ||
| + | !Colonies | ||
| + | |- | ||
| + | |align=center|lac promoter/rbs(34)+ rep + pSB1A2 | ||
| + | |align=center|0 | ||
| + | |- | ||
| + | |align=center|lac promoter/rbs(30)rep/oriV (6) + lac promoter/rbs(34)aada/oriT (A) +pSB1A3 | ||
| + | |align=center|113 | ||
| + | |- | ||
| + | |align=center|lac promoter/rbs(30)rep/oriV (6) + lac promoter/rbs(34)aada/oriT (A) | ||
| + | |align=center|141 | ||
| + | |- | ||
| + | |align=center|lac promoter/rbs(30)rep/oriV (6) + lac promoter/rbs(34)aada/oriT (B) +pSB1A3 | ||
| + | |align=center|150 | ||
| + | |- | ||
| + | |align=center|oriV (6) + oriT + pSB1A7 | ||
| + | |align=center|200 | ||
| + | |- | ||
| + | |align=center|pSB1A7 self (did not de-phosphorylate) | ||
| + | |align=center|50 | ||
| + | |- | ||
| + | |align=center|DH5-a with RP4 | ||
| + | |align=center|3 | ||
| + | |- | ||
| + | |align=center|DB3.1 with RP4 | ||
| + | |align=center|0 | ||
| + | |- | ||
| + | |align=center|(+) pUC18 | ||
| + | |align=center|lawn | ||
| + | |- | ||
| + | |align=center|(-) no plasmid | ||
| + | |align=center|0 | ||
| + | |} | ||
| + | |||
= Discussion = | = Discussion = | ||
Revision as of 03:25, 19 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of transformants
- Grace
| Construct | Colonies |
|---|---|
| nir + rbs+GFP+tt in BBpRL1383a-1 | 2 |
| plac + rbs+GFP+tt in BBpRL1383a-1 | 0 |
| J33207 in pRL1383a (all plates) | lawn |
- Colony PCR of transformants
- None of the colonies tested positive for their desired inserts
Transformation
- Margaret
| Construct | Colonies |
|---|---|
| lac promoter/rbs(34)+ rep + pSB1A2 | 0 |
| lac promoter/rbs(30)rep/oriV (6) + lac promoter/rbs(34)aada/oriT (A) +pSB1A3 | 113 |
| lac promoter/rbs(30)rep/oriV (6) + lac promoter/rbs(34)aada/oriT (A) | 141 |
| lac promoter/rbs(30)rep/oriV (6) + lac promoter/rbs(34)aada/oriT (B) +pSB1A3 | 150 |
| oriV (6) + oriT + pSB1A7 | 200 |
| pSB1A7 self (did not de-phosphorylate) | 50 |
| DH5-a with RP4 | 3 |
| DB3.1 with RP4 | 0 |
| (+) pUC18 | lawn |
| (-) no plasmid | 0 |
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
