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Team:Hawaii/Notebook/2008-10-17
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Restreaked BBpRL1383a construct=== :<strong> Grace</strong> :* Looked at J33207+pRL1383a (BBpRL1383a) plates. 50 μl...) |
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:* Looked at J33207+pRL1383a (BBpRL1383a) plates. 50 μl plate had blue colonies. Restreaked on LB+sp<sub>2.5</sub>+sm<sub>2.5</sub> + X-gal | :* Looked at J33207+pRL1383a (BBpRL1383a) plates. 50 μl plate had blue colonies. Restreaked on LB+sp<sub>2.5</sub>+sm<sub>2.5</sub> + X-gal | ||
| + | ===Inoculated for plasmid prep=== | ||
| + | :<strong>Grace</strong> | ||
| + | |||
| + | :* Inoculated 200 ml TB+sp<sub>2.5</sub>+sm<sub>2.5</sub> with blue colony. Incubated over the weekend at 37C with shaking at 250 rpm. | ||
= Discussion = | = Discussion = | ||
Revision as of 00:57, 18 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Restreaked BBpRL1383a construct
- Grace
- Looked at J33207+pRL1383a (BBpRL1383a) plates. 50 μl plate had blue colonies. Restreaked on LB+sp2.5+sm2.5 + X-gal
Inoculated for plasmid prep
- Grace
- Inoculated 200 ml TB+sp2.5+sm2.5 with blue colony. Incubated over the weekend at 37C with shaking at 250 rpm.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
