Team:Hawaii/Notebook/2008-10-26

From 2008.igem.org

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(Plasmid prep)
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::* ~2ng/μl
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::* The prep kit yields really clean preps in ~1 hour. :D!
::* The prep kit yields really clean preps in ~1 hour. :D!
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===Plasmid prep===
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[[Image:102508plasmid.png|right|thumb|EtBr stained 0.8% agarose gel ran at 95V for 1 hour. 5 microliters of plasmid were loaded into wells 3 and 4 while ligation products were loaded into lanes 5 and 6.]]
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:<strong> Margaret</strong>
= Discussion =
= Discussion =

Revision as of 01:31, 27 October 2008

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Things we did today

Wetlab work

Plasmid prep

EtBr stained 0.8% agarose gel ran at 95V for 1 hour. Ten microliters of plasmid were loaded into each well.
Grace
  • Used Qiaprep Spin Miniprep Kit to plasmid prep BB1 (10/22 and 10/25 inoculations)
  • Ran on gel to verify plasmid
  • ~2ng/μl
  • The prep kit yields really clean preps in ~1 hour. :D!

Plasmid prep

EtBr stained 0.8% agarose gel ran at 95V for 1 hour. 5 microliters of plasmid were loaded into wells 3 and 4 while ligation products were loaded into lanes 5 and 6.
Margaret

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]