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Team:Hawaii/Notebook/2008-10-28
From 2008.igem.org
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===[[Team:Hawaii/Antibiotic_test_for_BB-pRL1383a |Antibiotic test]]=== | ===[[Team:Hawaii/Antibiotic_test_for_BB-pRL1383a |Antibiotic test]]=== | ||
:<strong>Grace</strong> | :<strong>Grace</strong> | ||
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:* Ran HindIII/BamHI digested J33207 on gel | :* Ran HindIII/BamHI digested J33207 on gel | ||
:* Extracted from gel | :* Extracted from gel | ||
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:* Used 5 μl ligation reaction to transform 100 μl DH5α MCR cells (from Doug in SC lab) | :* Used 5 μl ligation reaction to transform 100 μl DH5α MCR cells (from Doug in SC lab) | ||
::* Also transformed 25 μl DH5α MCR with 2 μl BB-1 1025 and 1 μl J33207 each. | ::* Also transformed 25 μl DH5α MCR with 2 μl BB-1 1025 and 1 μl J33207 each. | ||
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| + | [[Image:PRL-K125820.jpg|right|thumb|Plate of BB-pRL1383a/K125820 transformants under blue light. Only some of the colonies appear to fluoresce and the smaller colonies do not show well in the picture.]] | ||
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| + | ===Checked for Transformants=== | ||
| + | :<strong>Krystle</strong> | ||
| + | |||
| + | :*BB-pRL1383a/K125820 produced a total of 27 transformants of varying size after incubation for 20 hours at 37<sup>o</sup>C | ||
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| + | ===Inoculate for Tri-Parental Conjugation=== | ||
| + | :<strong>Krystle</strong> | ||
| + | |||
| + | :*Inoculated 10ml cultures of LB with | ||
| + | :** BB-pRL1383a with sp<sub>100</sub> | ||
| + | :** BB-pRL1383a/K125820 with sp<sub>100</sub> | ||
| + | :** RP1 with kan<sub>50</sub> | ||
== Drylab Work == | == Drylab Work == | ||
Latest revision as of 03:41, 30 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Antibiotic test
- Grace
- Ran HindIII/BamHI digested J33207 on gel
- Extracted from gel
- Ligated with HindIII/BamHI digested pRL1383a
- Used 5 μl ligation reaction to transform 100 μl DH5α MCR cells (from Doug in SC lab)
- Also transformed 25 μl DH5α MCR with 2 μl BB-1 1025 and 1 μl J33207 each.
Checked for Transformants
- Krystle
- BB-pRL1383a/K125820 produced a total of 27 transformants of varying size after incubation for 20 hours at 37oC
Inoculate for Tri-Parental Conjugation
- Krystle
- Inoculated 10ml cultures of LB with
- BB-pRL1383a with sp100
- BB-pRL1383a/K125820 with sp100
- RP1 with kan50
- Inoculated 10ml cultures of LB with
Drylab Work
Project page
- Grace
- Wrote up Materials, Methods and Results for Project Part B on team project page.
Sequence analysis
- Grace
- Same G->C transversion in CAP binding site for BBpRL-1 sequence.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
