Team:Hawaii/Notebook/2008-10-28

From 2008.igem.org

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= Things we did today =
= Things we did today =
== Wetlab work ==
== Wetlab work ==
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===Ran RE digests on gel===
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:<strong> Grace</strong>
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[[Image:102808REdigest.jpg|right|thumb|EtBr stained 4% agarose gel ran at 60V for 3.5 hours. Ten microliters of XbaI/SpeI digested DNA were loaded into each lane.]]
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:* Gel picture for presentation
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===[[Team:Hawaii/Antibiotic_test_for_BB-pRL1383a |Antibiotic test]]===
===[[Team:Hawaii/Antibiotic_test_for_BB-pRL1383a |Antibiotic test]]===

Revision as of 22:32, 29 October 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Antibiotic test

Grace
File:102808j33207.jpg
EtBr stained 2% agarose gel ran at 60V for 2 hours. Twenty microliters of HindIII/BamHI digested J33207 were loaded.
  • Ran HindIII/BamHI digested J33207 on gel
  • Extracted from gel
  • Ligated with HindIII/BamHI digested pRL1383a
  • Used 5 μl ligation reaction to transform 100 μl DH5α MCR cells (from Doug in SC lab)
  • Also transformed 25 μl DH5α MCR with 2 μl BB-1 1025 and 1 μl J33207 each.

Drylab Work

Project page

Grace
  • Wrote up Materials, Methods and Results for Project Part B on team project page.

Sequence analysis

Grace
  • Same G->C transversion in CAP binding site for BBpRL-1 sequence.

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]