Team:Hawaii/Notebook/2008-10-28

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Revision as of 22:32, 29 October 2008 by Gracek (Talk | contribs)
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Things we did today

Wetlab work

Antibiotic test

Grace
File:102808j33207.jpg
EtBr stained 2% agarose gel ran at 60V for 2 hours. Twenty microliters of HindIII/BamHI digested J33207 were loaded.
  • Ran HindIII/BamHI digested J33207 on gel
  • Extracted from gel
  • Ligated with HindIII/BamHI digested pRL1383a
  • Used 5 μl ligation reaction to transform 100 μl DH5α MCR cells (from Doug in SC lab)
  • Also transformed 25 μl DH5α MCR with 2 μl BB-1 1025 and 1 μl J33207 each.

Drylab Work

Project page

Grace
  • Wrote up Materials, Methods and Results for Project Part B on team project page.

Sequence analysis

Grace
  • Same G->C transversion in CAP binding site for BBpRL-1 sequence.

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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