"
Team:Hawaii/Protocols
From 2008.igem.org
(Difference between revisions)
(→In vitro) |
|||
| Line 5: | Line 5: | ||
This is a list of tried and true protocols used by our team. | This is a list of tried and true protocols used by our team. | ||
===''In vitro''=== | ===''In vitro''=== | ||
| - | * Oligonucleotide hybridization | + | * [[Team:Hawaii/Protocols/oligo_hybridization|Oligonucleotide hybridization]] |
| - | * DNA ligation | + | * [[Team:Hawaii/Procols/DNA_ligation|DNA ligation]] |
* DNA restriction digest | * DNA restriction digest | ||
| + | * [[Team:Hawaii/Protocols/gel_extraction|Gel extraction]] | ||
===''E. coli''=== | ===''E. coli''=== | ||
Revision as of 21:49, 9 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
List of Protocols
This is a list of tried and true protocols used by our team.
In vitro
- Oligonucleotide hybridization
- DNA ligation
- DNA restriction digest
- Gel extraction
E. coli
- Cryopreserve cells
- Making competent cells
- Transformation of E. coli
- Alkaline lysis / plasmid isolation
Synechocystis sp. PCC6803
Media
- Solid growth media
- Liquid growth media
- Antibiotics
BioBrick
- BioBrick Construction Tutorial
- Silver:BioBrick Strategy
- Designing BioBrick Primers
- BioBrick Part Fabrication
