Team:Heidelberg/Notebook/Sensing Group/Notebook/1stweek

From 2008.igem.org

(Difference between revisions)
(Monday, 08/04/2008)
(Monday, 08/04/2008)
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=== Cloning of LuxP ===
=== Cloning of LuxP ===
* PCR of LuxP from ''V. harveyi'' colony
* PCR of LuxP from ''V. harveyi'' colony
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[[Image:HD_080805-LuxP_PCR.png|middle|300px]]
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[[Image:HD_080805-LuxP_PCR.png|middle|100px|LuxP colony PCR]]
== Tuesday, 08/05/2008 ==
== Tuesday, 08/05/2008 ==

Revision as of 09:44, 26 October 2008

Back to the overview

Contents

Monday, 08/04/2008

Preparations

Preparations were done at the end of the week before

  • Transformation of pDK48 and pTrc99a in E. coli DH5a (0.5 µl plasmid-DNA + 50 µl competent cells)
  • Glycerol-stock of Vibrio harveyi BB120, BB886, mm30, BB178, BB125
  • picked pTrc99a and pDK48 cultures and inoculated in 3 ml LB overnight
  • V. harveyi cultured on LB+-Agar plate
  • MiniPrep of pTrc99a and pDK48 from DHha

Cloning of LuxP

  • PCR of LuxP from V. harveyi colony

LuxP colony PCR

Tuesday, 08/05/2008

Wednesday, 08/06/2008

Thursday, 08/07/2008

Friday, 08/08/2008