Team:Heidelberg/Notebook/Sensing Group/Notebook/1stweek

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Contents

Monday, 08/04/2008

Preparations

Preparations were done at the end of the week before

  • Transformation of pDK48 and pTrc99a in E. coli DH5a (0.5 µl plasmid-DNA + 50 µl competent cells)
  • Glycerol-stock of Vibrio harveyi BB120, BB886, mm30, BB178, BB125
  • picked pTrc99a and pDK48 cultures and inoculated in 3 ml LB overnight
  • V. harveyi cultured on LB+-Agar plate
  • MiniPrep of pTrc99a and pDK48 from DHha

Cloning of LuxP

  • PCR of LuxP from V. harveyi colony

LuxP colony PCR

Tuesday, 08/05/2008

Wednesday, 08/06/2008

Thursday, 08/07/2008

Friday, 08/08/2008