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Team:Heidelberg/Notebook/Sensing Group/Notebook/3rdweek
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* PCR for LuxQ-1 and LuxQ-2 for Fusion constructs. Different Mg concentrations between 1-4 % were used. For LuxQ-2 another PCR was run using as template ''V. harveyi'' colonies | * PCR for LuxQ-1 and LuxQ-2 for Fusion constructs. Different Mg concentrations between 1-4 % were used. For LuxQ-2 another PCR was run using as template ''V. harveyi'' colonies | ||
[[Image:HD 080818-F1 LuxQ1 2.png|center|thumb|500px|PCR of LuxQ-1 and LuxQ-2 for subsequent fusion PCR]] | [[Image:HD 080818-F1 LuxQ1 2.png|center|thumb|500px|PCR of LuxQ-1 and LuxQ-2 for subsequent fusion PCR]] | ||
| + | * Fusion-PCR for Fusion-1 and Fusion-2, each 4 tubes (2 µl Tar, 1 µl LuxQ) | ||
| + | * preparation of O/N culture of LuxS and pDK48 transformed cells for subsequent miniprep | ||
| + | * digestion of pDK48 with NcoI/NdeI | ||
| + | * digestion of pTrc99alpha and LuxQ (with BamHI and NcoI) and gelextraction | ||
== Tuesday, 08/19/2008 == | == Tuesday, 08/19/2008 == | ||
Revision as of 14:15, 26 October 2008
Contents |
Monday, 08/18/2008
- PCR for LuxQ-1 and LuxQ-2 for Fusion constructs. Different Mg concentrations between 1-4 % were used. For LuxQ-2 another PCR was run using as template V. harveyi colonies
- Fusion-PCR for Fusion-1 and Fusion-2, each 4 tubes (2 µl Tar, 1 µl LuxQ)
- preparation of O/N culture of LuxS and pDK48 transformed cells for subsequent miniprep
- digestion of pDK48 with NcoI/NdeI
- digestion of pTrc99alpha and LuxQ (with BamHI and NcoI) and gelextraction
