Team:Heidelberg/Notebook/Sensing Group/Notebook/4thweek

From 2008.igem.org

(Difference between revisions)
(Monday, 08/25/2008)
(Monday, 08/25/2008)
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== Monday, 08/25/2008 ==
== Monday, 08/25/2008 ==
-
[[Image:HD 080826-pDK48 digestions.png|right|thumb|200px|Digestion of pDK48 with xbaI and PstI]]
+
[[Image:HD 080826-pDK48 digestions.png|right|thumb|200px|Digestion of pDK48 with xbaI and PstI yield expected results]]
* sequentiell and simultaneous digestion of pDk48 NcoI/NdeI and NcoI/KpnI
* sequentiell and simultaneous digestion of pDk48 NcoI/NdeI and NcoI/KpnI
--> not successful
--> not successful

Revision as of 14:52, 26 October 2008

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Contents

Monday, 08/25/2008

Digestion of pDK48 with xbaI and PstI yield expected results
  • sequentiell and simultaneous digestion of pDk48 NcoI/NdeI and NcoI/KpnI

--> not successful

  • preparation of O/N culture of pDK48 transformed cells
  • digestion of pDK48 with XbaI and PstI to check wether the vector is the right one


Tuesday, 08/26/2008

Wednesday, 08/27/2008

Thursday, 08/28/2008

Friday, 08/29/2008

Go to 5th week