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Team:Heidelberg/Notebook/Sensing Group/Notebook/4thweek
From 2008.igem.org
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== Tuesday, 08/26/2008 == | == Tuesday, 08/26/2008 == | ||
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| + | === Cloning === | ||
[[Image:HD 080826-pDK48 digestions.png|right|thumb|200px|Digestion of pDK48 with xbaI and PstI yield expected results]] | [[Image:HD 080826-pDK48 digestions.png|right|thumb|200px|Digestion of pDK48 with xbaI and PstI yield expected results]] | ||
* digestion of pDK48 with XbaI and PstI to check wether the vector is the right one | * digestion of pDK48 with XbaI and PstI to check wether the vector is the right one | ||
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| + | === LuxS Test === | ||
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<br style="clear: both;"/> | <br style="clear: both;"/> | ||
Revision as of 14:57, 26 October 2008
Contents |
Monday, 08/25/2008
- sequentiell and simultaneous digestion of pDk48 NcoI/NdeI and NcoI/KpnI
--> not successful
- preparation of O/N culture of pDK48 transformed cells
Tuesday, 08/26/2008
Cloning
- digestion of pDK48 with XbaI and PstI to check wether the vector is the right one
LuxS Test
