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Team:KULeuven/22 July 2008
From 2008.igem.org
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Contents |
Lab Work
Wet Lab
Today, the transformed bacteria arrived from MIT. These were plated out and will grow overnight. Five transformed bacteria (M30109, B0032, E0022, B0015 and R0084) were inoculated in liquid culture. Tomorrow, we will make a liquid culture and glycerol stock from the rest of them. The competent cells made on Friday seemed to have grown.
Modeling
We managed our first day without Jonas...
Nick and Maarten worked on the cascade linking of the filter with the invertor. The variation in LuxR is too insensitive to the input of light to make a difference in the variation in CcdB, cell death seems to occur always. This problem should be solved by investigating the parameters thoroughly.
Memory seems to fail, the promotor's strength is not high enough to keep the memory digital value to 0, thereby keeping the system in its off state when no light is shed on the system... eventually the memory will change its state to 1, light or not.
The modeling wiki has been worked on and under construction to make it readable to other teams. Current goal is to retrieve the information used to determine the parameters. Some have been updated, the parameters for the pulse generator have been added...
Remarks
A press notice was written and will soon be released.
The sun shines. Finally.
