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Team:KULeuven/22 July 2008
From 2008.igem.org
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Contents |
Lab Work
Wet Lab
Today, the transformed bacteria arrived from MIT. These were plated out and will grow overnight. Five transformed bacteria ([http://partsregistry.org/Part:BBa_M30109 M30109], [http://partsregistry.org/Part:BBa_B0032 B0032], [http://partsregistry.org/Part:BBa_E0022 E0022], [http://partsregistry.org/Part:BBa_B0015 B0015] and [http://partsregistry.org/Part:BBa_R0084 R0084]) were also inoculated in liquid LB medium. Tomorrow, we will make a liquid culture and glycerol stock from the other parts. The competent cells made on Friday seemed to have grown.
Dry Lab
Modeling
We managed our first day without Jonas...
Nick and Maarten worked on the cascade linking of the filter with the inverter. There are serious problems with linking these 2 parts: in a normal scenario there's an equilibrium between LuxR and the HSL_LuxR-complex in which the amount of LuxR is higher than the amount of the complex. This could be fixed by finetuning some parameters (decreasing/increasing some of the strengths of the promoters or the efficiencies of the rbs'). But the main problem remains: in the equilibrium there's still a vast amount of LuxR which represses the transcription of ccdB. This way, cell death can never take place.
Memory seems to fail, the promoter's strength is not high enough to keep the memory's digital value at 0, thereby keeping the system in its off state when no light is shed on the system... After some time the memory will always change its state to 1, light or not.
The modeling wiki has been worked on and is under construction to make it readable to other teams. Current goal is to retrieve the information used to determine the parameters. Some have been updated, the parameters for the pulse generator and the inverter have been added...
Other
A press notice was written and will soon be released.
Remarks
The sun is shining. Finally.
