Team:KULeuven/4 August 2008

From 2008.igem.org

(Difference between revisions)
(Wet Lab)
(Wet Lab)
Line 5: Line 5:
=== Wet Lab ===
=== Wet Lab ===
-
* Competent cells were inoculated and prepared for elektroporation with the folowing ligation products: C0040+B0015, J23109+J23032, I712074+J23032, E0022+B0015, P1010+B0015, C0060+B0015, C0062+B0015, R0084+B0032,... The elektroporation was performed and hopefully we will have transformed cells tomorrow.
+
* Competent cells were inoculated and prepared for electroporation with the folowing ligation products: C0040+B0015, J23109+J23032, I712074+J23032, E0022+B0015, P1010+B0015, C0060+B0015, C0062+B0015, R0084+B0032, J23100+B0032. The electroporation was performed and hopefully we will have transformed cells tomorrow.
 +
 
 +
* We continued to make the GFP-LVA Biobrick. The PCR product was put on gel and purified. It gave nice results,so the PCR worked well. Tomorrow we will cut it with ''Eco''RI and ''Spe''I.
* We made electrocompetent cells and tested them with pUC. Tomorrow we'll see if they're any good. A glycerolstock of the cells has been stored in the -80° freezer.
* We made electrocompetent cells and tested them with pUC. Tomorrow we'll see if they're any good. A glycerolstock of the cells has been stored in the -80° freezer.

Revision as of 08:54, 5 August 2008

<< return to notebook return to homepage >>
< previous friday ← yesterday tomorrow → next monday >

Contents

Lab Work

Wet Lab

  • Competent cells were inoculated and prepared for electroporation with the folowing ligation products: C0040+B0015, J23109+J23032, I712074+J23032, E0022+B0015, P1010+B0015, C0060+B0015, C0062+B0015, R0084+B0032, J23100+B0032. The electroporation was performed and hopefully we will have transformed cells tomorrow.
  • We continued to make the GFP-LVA Biobrick. The PCR product was put on gel and purified. It gave nice results,so the PCR worked well. Tomorrow we will cut it with EcoRI and SpeI.
  • We made electrocompetent cells and tested them with pUC. Tomorrow we'll see if they're any good. A glycerolstock of the cells has been stored in the -80° freezer.

Dry Lab

Modeling

Wiki

Remarks