Team:Missouri Miners/Project

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== '''Overall project''' ==
== '''Overall project''' ==
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The goal of this research is the manipulation of yeast cells; granting them the capability of measuring the concentration of ethanol present. This project utilizes the metabolic pathways of the yeast Pichia pastoris, which are capable of metabolizing ethanol and methanol. The enzyme, alcohol oxidase (AO), encoded in the AOXI gene appears to be the major enzyme involved in methanol metabolism.  If both carbon sources are present, however, P. pastoris prefers to utilize ethanol first. This preference is controlled by the AOXI promoter.  Fusing the AOXI promoter with a fluorescent protein gene will allow visible detection of the expression of AOXI. In supplying the yeast with ethanol and methanol simultaneously, the cells should produce the fluorescent protein after ethanol consumption; resulting in a visible color and fluorescence. The concentration of ethanol can be determined by measuring the time before fluorescence and in doing so, will make plausible the development of a breathalyzer device and additional sensor systems.
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== Project Details==
== Project Details==

Revision as of 00:51, 1 August 2008


This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have a team description page, a project abstract, a complete project description, and a lab notebook. PLEASE keep all of your pages within your Team:Example namespace.



You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
Example logo.png

Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)

Your team picture
Team Example 2


Home The Team The Project Parts Submitted to the Registry Modeling Notebook

(Or you can choose different headings. But you must have a team page, a project page, and a notebook page.)


Contents

Overall project

The goal of this research is the manipulation of yeast cells; granting them the capability of measuring the concentration of ethanol present. This project utilizes the metabolic pathways of the yeast Pichia pastoris, which are capable of metabolizing ethanol and methanol. The enzyme, alcohol oxidase (AO), encoded in the AOXI gene appears to be the major enzyme involved in methanol metabolism. If both carbon sources are present, however, P. pastoris prefers to utilize ethanol first. This preference is controlled by the AOXI promoter. Fusing the AOXI promoter with a fluorescent protein gene will allow visible detection of the expression of AOXI. In supplying the yeast with ethanol and methanol simultaneously, the cells should produce the fluorescent protein after ethanol consumption; resulting in a visible color and fluorescence. The concentration of ethanol can be determined by measuring the time before fluorescence and in doing so, will make plausible the development of a breathalyzer device and additional sensor systems.

Project Details

Part 2

The Experiments

Part 3

Results