Team:Montreal/DNA Agarose Gel

From 2008.igem.org

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=='''Procedure'''==
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==Procedure==
1. Measure 50mL of TAE buffer and mix with 0.5g of Agarose powder.
1. Measure 50mL of TAE buffer and mix with 0.5g of Agarose powder.

Revision as of 03:30, 17 June 2008

Home The Team The Project Parts Submitted to the Registry Modeling Notebook


Procedure

1. Measure 50mL of TAE buffer and mix with 0.5g of Agarose powder.

2. Thoroughly mix the two in the flask and microwave the mixture for roughly about 1 minute (or until you see bubbling).

3. Add 1.5µL of Ethidium Bromide and swirl until a fine mixture is seen.

4. Pour the mixture onto the casting tray (make sure that each end is taped), with a comb inserted in one of the ends.

5. Wait for the gel to solidify over time.

6. Once hardened, remove the comb and load the gel into the electrophoresis box.

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