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Team:Paris/August 21
From 2008.igem.org
(Difference between revisions)
(New page: ='''Construction of pFlgA - YFP tripart (+/- LVA)'''= Aim : Construction of ''' "pFlgA-RBS-YFP-dbl ter" (pFlgA-E0430/E0432)''' Image:Part_icon_regulatory.png[[Image:Part_icon_rbs.png]...) |
(→Gel Extraction) |
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| Line 27: | Line 27: | ||
|MP165.1 | |MP165.1 | ||
|MP166.1 | |MP166.1 | ||
| - | |||
|no sample | |no sample | ||
|D166 | |D166 | ||
|no sample | |no sample | ||
|D167 | |D167 | ||
| - | |||
| - | |||
|no sample | |no sample | ||
|100pb ladder | |100pb ladder | ||
| + | |colspan="3"|no sample | ||
|- | |- | ||
|'''Expected size (pb)''' | |'''Expected size (pb)''' | ||
| Line 41: | Line 39: | ||
|2 957 | |2 957 | ||
|2 996 | |2 996 | ||
| - | |||
| | | | ||
| - | | | + | |2942 |
| - | + | ||
| - | + | ||
| | | | ||
| + | |2981 | ||
| | | | ||
|- | |- | ||
|'''Measured size (pb)''' | |'''Measured size (pb)''' | ||
| | | | ||
| - | |||
|style="background: #cbff7B"| 3 000 | |style="background: #cbff7B"| 3 000 | ||
|style="background: #cbff7B"| 3 000 | |style="background: #cbff7B"| 3 000 | ||
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|style="background: #cbff7B"| | |style="background: #cbff7B"| | ||
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Revision as of 10:28, 21 August 2008
Construction of pFlgA - YFP tripart (+/- LVA)
Aim : Construction of "pFlgA-RBS-YFP-dbl ter" (pFlgA-E0430/E0432) 
Digestion
Gel Extraction
Protocol [[Image:KR00019b.jpg| thumb|Gel Extraction of D166-D167]
| Well | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| Sample | 1kb ladder | MP165.1 | MP166.1 | no sample | D166 | no sample | D167 | no sample | 100pb ladder | no sample | ||
| Expected size (pb) | 2 957 | 2 996 | 2942 | 2981 | ||||||||
| Measured size (pb) | 3 000 | 3 000 | ||||||||||
