Team:Paris/Modeling/Molecular Reactions

From 2008.igem.org

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<html><iframe height="300" width="600" src="https://static.igem.org/mediawiki/2008/3/3a/Complexation_Reactions.pdf"></iframe></html>
<html><iframe height="300" width="600" src="https://static.igem.org/mediawiki/2008/3/3a/Complexation_Reactions.pdf"></iframe></html>
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| width="25%" | '''On the Example''' <br>
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| width="25%" | '''On the Example :''' <br>
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How given amounts of ''FlhDC'' and ''FliA'' would produce ''FP1'' ? <br>
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<br>
The complexation of ''FlhDC'' on ''pFliL'' : <br>
The complexation of ''FlhDC'' on ''pFliL'' : <br>
[[Image:DCiLcompl.jpg]]
[[Image:DCiLcompl.jpg]]
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<html><iframe height="300" width="600" src="https://static.igem.org/mediawiki/2008/e/ef/Production_Reactions.pdf"></iframe></html>
<html><iframe height="300" width="600" src="https://static.igem.org/mediawiki/2008/e/ef/Production_Reactions.pdf"></iframe></html>
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| width="25%" | '''On the Example''' <br>
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| width="25%" | '''On the Example :''' <br>
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The production of ''FP1'' by ''FlhDC><pFliL'' and ''FliA><pFliL'' : <br>
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How given amounts of ''FlhDC'' and ''FliA'' would produce ''FP1'' ? <br>
 +
<br>
 +
''FlhDC><pFliL'' and ''FliA><pFliL'' causes "production" of ''FP1'' : <br>
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<br> <br>
[[Image:bFP1.jpg]]
[[Image:bFP1.jpg]]
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<html><iframe height="300" width="600" src="https://static.igem.org/mediawiki/2008/f/f7/Degradation_Dilution.pdf"></iframe></html>
<html><iframe height="300" width="600" src="https://static.igem.org/mediawiki/2008/f/f7/Degradation_Dilution.pdf"></iframe></html>
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| width="25%" | '''On the Example''' <br>
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| width="25%" | '''On the Example :''' <br>
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The disappearance of ''FP1'' because of its degradation/dilution : <br>
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How given amounts of ''FlhDC'' and ''FliA'' would produce ''FP1'' ? <br>
 +
<br>
 +
''FP1'' disappears because of its degradation and dilution : <br>
[[Image:dFP1.jpg]]
[[Image:dFP1.jpg]]

Revision as of 19:43, 26 October 2008

Molecular Reactions



We show here the elementary reactions from wich we started. They intend to be "exhaustives" in the sense that every steps of the System is described. Each of those reactions has to be "mathematically interpreted", in a Model (see the model) that accounts for the important phenomenon, and in the same time allows us to find experimentally (see the protocol) every involved parameters.


The System we are studying is the "Core System" (see Network Design) that we can represent in the following, with all alternatives (pFlhDC/pTet ; TetR/EnvZ/OmpR*) :
The Full System
The interaction surrounded in red (f5) is the one of the detailed example


Complexation Reactions

The double triangle (|><|) is a symbol for complexations :
Compl.jpg means n1 molecules of FlhDC complexed with a pFliA promoter

On the Example :

How given amounts of FlhDC and FliA would produce FP1 ?

The complexation of FlhDC on pFliL :
DCiLcompl.jpg The complexation of FliA on pFliL :
AiLcompl.jpg

Production Reactions

The double triangle (|><|) is a symbol for complexations :
Compl.jpg means n1 molecules of FlhDC complexed with a pFliA promoter

On the Example :

How given amounts of FlhDC and FliA would produce FP1 ?

FlhDC><pFliL and FliA><pFliL causes "production" of FP1 :


BFP1.jpg

Degradation/Dilution Reactions

On the Example :

How given amounts of FlhDC and FliA would produce FP1 ?

FP1 disappears because of its degradation and dilution :
DFP1.jpg


for complete .pdf with all reactions and references : [list of reactions/equations]