"
Team:Paris/Modeling/Workflow Example
From 2008.igem.org
(Difference between revisions)
| Line 3: | Line 3: | ||
Here, we will explain the generic protocol that leads to the characterisation of one of the steps of our system. The choosen example is '''the EXPRESSION of the PROMOTER pFliL in function of the TRANSCRIPTION FACTORS FlhDC and FliA'''. | Here, we will explain the generic protocol that leads to the characterisation of one of the steps of our system. The choosen example is '''the EXPRESSION of the PROMOTER pFliL in function of the TRANSCRIPTION FACTORS FlhDC and FliA'''. | ||
| - | We know that ''the hexamere FlhD<sub>4</sub>C<sub>2</sub>'' and the protein ''FliA'' act both on the promoter ''pFliL'' as inducers... how do we evaluate this | + | We know that ''the hexamere FlhD<sub>4</sub>C<sub>2</sub>'' and the protein ''FliA'' act both on the promoter ''pFliL'' as inducers... how do we evaluate this phenomenon? |
| - | + | Starting from [[Team:Paris/Modeling/Molecular_Reactions|molecular reactions]] '''(1)''', we develop a [[Team:Paris/Modeling/FromMolReactToNLOde|model of our system]] '''(2)''', and then we identify the parameters '''(3)''' that needs to be estimated. Essentially, it amounts to estimate [[Team:Paris/Modeling/Protocol_Of_Characterisation|Promoters Activities as functions of their Transcription Factors]]. To do so, we propose [[Team:Paris/Modeling/Protocol_Of_Characterisation|constructs]] '''(4)''', [[Team:Paris/Modeling/Protocol_Of_Characterisation|protocols]] '''(5)''' and [[Team:Paris/Modeling/Implementation|programs]] '''(6)'''. | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | Starting from [[Team:Paris/Modeling/Molecular_Reactions|molecular reactions]] '''(1)''', we develop a [[Team:Paris/Modeling/FromMolReactToNLOde|model of our system]] '''(2)''', we | + | |
<br> | <br> | ||
Revision as of 14:35, 26 October 2008
|
Generic Workflow on an Example
Here, we will explain the generic protocol that leads to the characterisation of one of the steps of our system. The choosen example is the EXPRESSION of the PROMOTER pFliL in function of the TRANSCRIPTION FACTORS FlhDC and FliA. We know that the hexamere FlhD4C2 and the protein FliA act both on the promoter pFliL as inducers... how do we evaluate this phenomenon? Starting from molecular reactions (1), we develop a model of our system (2), and then we identify the parameters (3) that needs to be estimated. Essentially, it amounts to estimate Promoters Activities as functions of their Transcription Factors. To do so, we propose constructs (4), protocols (5) and programs (6).
|
