Team:Paris/Modeling/f3bis

From 2008.igem.org

(Difference between revisions)
Line 63: Line 63:
|value
|value
|-
|-
-
|β<sub>12</sub>
+
|β<sub>12bis</sub>
-
|production rate of FliA-pFlhDC '''with RBS E0032''' <br> β<sub>12</sub>
+
|production rate of FliA-pFlhDC '''with RBS E0032''' <br> replace β<sub>12</sub> (not written)
|nM.s<sup>-1</sup>
|nM.s<sup>-1</sup>
|
|
|-
|-
-
|(K<sub>11</sub>/{coef<sub>fliA</sub>}<sup>n<sub>11</sub></sup>)
+
|(K<sub>11bis</sub>/{coef<sub>fliA</sub>}<sup>n<sub>11bis</sub></sup>)
-
|activation constant of FliA-pFlhDC <br> K<sub>11</sub>
+
|activation constant of FliA-pFlhDC <br> K<sub>11bis</sub>
-
|nM<sup>n<sub>11</sub></sup>
+
|nM<sup>n<sub>11bis</sub></sup>
|
|
|-
|-
-
|n<sub>11</sub>
+
|n<sub>11bis</sub>
-
|complexation order of FliA-pFlhDC <br> n<sub>11</sub>
+
|complexation order of FliA-pFlhDC <br> replace n<sub>11</sub> (not written)
|no dimension
|no dimension
|
|
|-
|-
|-
|-
-
|β<sub>2</sub>
+
|β<sub>2bis</sub>
-
|production rate of OmpR-pFlhDC '''with RBS E0032''' <br> β<sub>2</sub>
+
|production rate of EnvZ-pFlhDC '''with RBS E0032''' <br> β<sub>2bis</sub>
|nM.s<sup>-1</sup>
|nM.s<sup>-1</sup>
|
|
|-
|-
-
|(K<sub>19</sub>/{coef<sub>ompR</sub>}<sup>n<sub>19</sub></sup>)
+
|(K<sub>19bis</sub>/{coef<sub>envZ</sub>}<sup>n<sub>19bis</sub></sup>)
-
|activation constant of OmpR-pFlhDC <br> K<sub>19</sub>
+
|activation constant of EnvZ-pFlhDC <br> replace K<sub>19</sub> (not written)
-
|nM<sup>n<sub>19</sub></sup>
+
|nM<sup>n<sub>19bis</sub></sup>
|
|
|-
|-
-
|n<sub>19</sub>
+
|n<sub>19bis</sub>
-
|complexation order of OmpR-pFlhDC <br> n<sub>19</sub>
+
|complexation order of OmpR-pFlhDC <br> n<sub>19bis</sub>
|no dimension
|no dimension
|
|
Line 99: Line 99:
Then, if we have time, we want to verify the expected relation
Then, if we have time, we want to verify the expected relation
-
<center>[[Image:SumFlhDC1.jpg]]</center>
+
<center>[[Image:SumpFlhDC2.jpg]]</center>

Revision as of 15:46, 21 August 2008

F3env.png
At the steady-state, we have
FliAeq.jpg

and

EnvZeq.jpg

so the expression

F3envexpr.jpg

gives

F3bisFliA.jpg

and

F3bisEnvZ.jpg

and for calculated values of the TF,

F3bisFliACalc.jpg

and

F3bisEnvZCalc.jpg



param signification unit value
[expr(pFlhDC)] expression rate of
pFlhDC with RBS E0032
nM.s-1 see "findparam"
need for 20 + 20 measures
and 5x5 measures for the SUM?
γGFP dilution-degradation rate
of GFP(mut3b)
s-1 ln(2)/3600
[GFP] GFP concentration at steady-state nM need for 20 + 20 measures
and 5x5 measures for the SUM?
(fluorescence) value of the observed fluorescence au need for 20 + 20 measures
and 5x5 measures for the SUM?
conversion conversion ration between
fluorescence and concentration
nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations
unit value
β12bis production rate of FliA-pFlhDC with RBS E0032
replace β12 (not written)
nM.s-1
(K11bis/{coeffliA}n11bis) activation constant of FliA-pFlhDC
K11bis
nMn11bis
n11bis complexation order of FliA-pFlhDC
replace n11 (not written)
no dimension
β2bis production rate of EnvZ-pFlhDC with RBS E0032
β2bis
nM.s-1
(K19bis/{coefenvZ}n19bis) activation constant of EnvZ-pFlhDC
replace K19 (not written)
nMn19bis
n19bis complexation order of OmpR-pFlhDC
n19bis
no dimension



Then, if we have time, we want to verify the expected relation

SumpFlhDC2.jpg