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Team:Paris/Modeling/f3bis
From 2008.igem.org
and
so the expression
gives
and
and for calculated values of the TF,
and
| param | signification | unit | value |
| [expr(pFlhDC)] | expression rate of pFlhDC with RBS E0032 | nM.s-1 | see "findparam" need for 20 + 20 measures and 5x5 measures for the SUM? |
| γGFP | dilution-degradation rate of GFP(mut3b) | s-1 | ln(2)/3600 |
| [GFP] | GFP concentration at steady-state | nM | need for 20 + 20 measures and 5x5 measures for the SUM? |
| (fluorescence) | value of the observed fluorescence | au | need for 20 + 20 measures and 5x5 measures for the SUM? |
| conversion | conversion ration between fluorescence and concentration | nM.au-1 | (1/79.429) |
| param | signification corresponding parameters in the equations | unit | value |
| β12bis | production rate of FliA-pFlhDC with RBS E0032 replace β12 (not written) | nM.s-1 | |
| (K11bis/{coeffliA}n11bis) | activation constant of FliA-pFlhDC K11bis | nMn11bis | |
| n11bis | complexation order of FliA-pFlhDC replace n11 (not written) | no dimension | |
| β2bis | production rate of EnvZ-pFlhDC with RBS E0032 β2bis | nM.s-1 | |
| (K19bis/{coefenvZ}n19bis) | activation constant of EnvZ-pFlhDC replace K19 (not written) | nMn19bis | |
| n19bis | complexation order of EnvZ-pFlhDC n19bis | no dimension |
Then, if we have time, we want to verify the expected relation
