"

 

From 2008.igem.org

(see the considerations on the use of EnvZ)

We have [EnvZ]_produced = {coef_env}expr(pTet) = {coef_env} ƒ1([aTc]_i)

and [EnvZ]_total = [EnvZ]_b + [EnvZ]_produced

and [FliA] = {coef_FliA}expr(pBad) = {coef_FliA} ƒ2([arab]_i)

So, if we denote phosphorylated OmpR by OmpR^*, we have

that we can then introduce in the previous expression (see ƒ3) :

param	signification	unit	value	comments
[expr(pFlhDC)]	expression rate of pFlhDC with RBS E0032	nM.min-1		need for 20 mesures with well choosen values of [aTc]i
γGFP	dilution-degradation rate of GFP(mut3b)	min-1	0.0198
[GFP]	GFP concentration at steady-state	nM		need for 20
(fluorescence)	value of the observed fluorescence	au		need for 20
conversion	conversion ratio between fluorescence and concentration	nM.au-1	(1/79.429)

param	signification corresponding parameters in the equations	unit	value	comments
K21eff	dissociation constant OmpR_-_EnvZ K21eff	nM		the literature [?] gives K21 =
n21	complexation order OmpR_-_EnvZ n21	no dimension		the literature [?] gives n21 =
{coefenv}	coefficient due to the difference of the RBS and degradation rate between EnvZ and GFP  ! not precised in the equations !	no dimension		! not precised in the equations ! watch out to write the corresponding simulating program
[EnvZ]b	"basal" presence of EnvZ [EnvZ]b	nM		the literature [?] gives, under high osmolarity, [EnvZ]b =
[OmpR]b	"basal" presence of OmpR [OmpR]b	nM		the literature [?] gives, under high osmolarity, [OmpR]b =

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