Team:Paris/Notebook/Oligo

From 2008.igem.org

(Difference between revisions)
Line 15: Line 15:
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|- style="background: #cccccc;"
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| style="background: #ccffcc;" | O101
| style="background: #ccffcc;" | O101
Line 20: Line 21:
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAACAGTATCGCGATGATCGCCACGCTACGT
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAACAGTATCGCGATGATCGCCACGCTACGT
| 58
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 26: Line 28:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGACAGTATCGCGATGATCGCCACGCTACG
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGACAGTATCGCGATGATCGCCACGCTACG
| 58
| 58
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 32: Line 35:
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGCATATCTCCTCCGCAGGTATCAAAATT
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGCATATCTCCTCCGCAGGTATCAAAATT
| 58
| 58
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 38: Line 42:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGCAGATGAAATCGATAAGCGACAAGG
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGCAGATGAAATCGATAAGCGACAAGG
| 58
| 58
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 44: Line 49:
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAAACGCCGCCTGGGCCGCGTTCAGTCCGCT
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAAACGCCGCCTGGGCCGCGTTCAGTCCGCT
| 58
| 58
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 50: Line 56:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCGGCGTTGTTGATGCAGATGGGAATA
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCGGCGTTGTTGATGCAGATGGGAATA
| 58
| 58
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 56: Line 63:
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACGAAGTGCGATCAATACTCATGGTTTATT
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACGAAGTGCGATCAATACTCATGGTTTATT
| 58
| 58
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 62: Line 70:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCACGTCATATCAGGCGGTCTGATAAGG
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCACGTCATATCAGGCGGTCTGATAAGG
| 58
| 58
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 68: Line 77:
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAGTTTTGTCGTCGCTCTCGTCAGACACGTC
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAGTTTTGTCGTCGCTCTCGTCAGACACGTC
| 58
| 58
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 74: Line 84:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTTGTATGTGCGTGTAGTGACGAGTACAG
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTTGTATGTGCGTGTAGTGACGAGTACAG
| 58
| 58
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 80: Line 91:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTCATTTTTGCTTGCTAGCGTACGGAAAA
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTCATTTTTGCTTGCTAGCGTACGGAAAA
| 58
| 58
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 86: Line 98:
| GTTTCTTCCTGCAGCGGCCGCTACTAGTATCCCACCCAGAATAACCAACTTTAT
| GTTTCTTCCTGCAGCGGCCGCTACTAGTATCCCACCCAGAATAACCAACTTTAT
| 54
| 54
 +
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 92: Line 105:
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGAATAACCAACTTTATTTTTATG
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGAATAACCAACTTTATTTTTATG
| 54
| 54
 +
|
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 98: Line 112:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCTGATTAACTGAGACTGACGGCAACGC
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCTGATTAACTGAGACTGACGGCAACGC
| 58
| 58
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 104: Line 119:
| CAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCCGCAGGGTTTTTAATCG
| CAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCCGCAGGGTTTTTAATCG
| 59
| 59
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 110: Line 126:
| GAAACGGATAATCATGCCGATAACTCATATAACGCAGGGCTG
| GAAACGGATAATCATGCCGATAACTCATATAACGCAGGGCTG
| 42
| 42
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 116: Line 133:
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACCATTACACCTTCAGCGGTATAGAGTGAACTCACGATAAA
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACCATTACACCTTCAGCGGTATAGAGTGAACTCACGATAAA
| 69
| 69
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 122: Line 140:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCAGCGATGAAATACTTGCCATGCGATT
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCAGCGATGAAATACTTGCCATGCGATT
| 58
| 58
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 128: Line 147:
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGCGAGAGGCTGTTGGTATTAATGACTT
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGCGAGAGGCTGTTGGTATTAATGACTT
| 58
| 58
 +
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 134: Line 154:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGTCGCATTCATCGCGCACCTCGTGGCTG
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGTCGCATTCATCGCGCACCTCGTGGCTG
| 58
| 58
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 140: Line 161:
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACTGGCTGATAACCCCTTCAATCCCCTGTA
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACTGGCTGATAACCCCTTCAATCCCCTGTA
| 58
| 58
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 146: Line 168:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGGCTGATAACCCCTTCAATCCCCTGT
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGGCTGATAACCCCTTCAATCCCCTGT
| 58
| 58
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 152: Line 175:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGGCTGATAACCCCTTCAATCCCCTGT
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGGCTGATAACCCCTTCAATCCCCTGT
| 58
| 58
 +
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 158: Line 182:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGCGAGAGGCTGTTGGTATTAATGACT
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGCGAGAGGCTGTTGGTATTAATGACT
| 58
| 58
 +
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 164: Line 189:
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACCAGCGATGAAATACTTGCCATGCGATTT
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACCAGCGATGAAATACTTGCCATGCGATTT
| 58
| 58
 +
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 170: Line 196:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGGCGATTGCGCTTCTCGCCAC
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGGCGATTGCGCTTCTCGCCAC
| 53
| 53
 +
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 176: Line 203:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTACCCTTCTTTTGTCGTGCCCTGCGCC
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTACCCTTCTTTTGTCGTGCCCTGCGCC
| 59
| 59
 +
|
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 182: Line 210:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTATTGATCGCACTTCGCCTC
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTATTGATCGCACTTCGCCTC
| 53
| 53
 +
|
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 188: Line 217:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGTTACTCTGCAAGTCTTGCTGCGCT
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGTTACTCTGCAAGTCTTGCTGCGCT
| 59
| 59
 +
|
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 194: Line 224:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTGAAAAAAGCATTGTTCAGG
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTGAAAAAAGCATTGTTCAGG
| 53
| 53
 +
|
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 200: Line 231:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAAACAGCCTGTACTCTCTGTTCATCC
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAAACAGCCTGTACTCTCTGTTCATCC
| 59
| 59
 +
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 206: Line 238:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTGAAAC
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTGAAAC
| 53
| 53
 +
|
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 212: Line 245:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGGCCCTTTTCTTGCGCAGCGCTTCT
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGGCCCTTTTCTTGCGCAGCGCTTCT
| 59
| 59
 +
|
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 218: Line 252:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGACCGCATAAAAATAAAGTTGGTTAT
| GTTTCTTCGAATTCGCGGCCGCTTCTAGACCGCATAAAAATAAAGTTGGTTAT
| 53
| 53
 +
|
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 224: Line 259:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATTCACTCTATACCGCTGAAG
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATTCACTCTATACCGCTGAAG
| 53
| 53
 +
|
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 230: Line 266:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTATAACTTACCCAGTTTAGTGCGTAAC
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTATAACTTACCCAGTTTAGTGCGTAAC
| 59
| 59
 +
|
 +
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 236: Line 274:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTAATCATGCCGATAACTCATATAAC
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTAATCATGCCGATAACTCATATAAC
| 53
| 53
 +
|
|
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 242: Line 281:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAAGAGAACTACAAGATTCTGG
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAAGAGAACTACAAGATTCTGG
| 53
| 53
 +
|
|
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|- style="background: #cccccc;"
|- style="background: #cccccc;"
Line 248: Line 288:
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTATGCTTTAGAGCCGTCCGGTACAAAG
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTATGCTTTAGAGCCGTCCGGTACAAAG
| 59
| 59
 +
|
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|}
|}

Revision as of 11:36, 30 July 2008

Table of Oligos
Number Name Sequence Length Tm(°C) Comments
O100 FlgA-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCATATCTCCTCCGCAGGTATCAAAAT 58
O101 FlgA-R GTTTCTTCCTGCAGCGGCCGCTACTAGTAACAGTATCGCGATGATCGCCACGCTACGT 58
O102 FlgB-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGACAGTATCGCGATGATCGCCACGCTACG 58
O103 FlgB-R GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGCATATCTCCTCCGCAGGTATCAAAATT 58
O104 FlgK-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGCAGATGAAATCGATAAGCGACAAGG 58
O105 FlgK-R GTTTCTTCCTGCAGCGGCCGCTACTAGTAAACGCCGCCTGGGCCGCGTTCAGTCCGCT 58
O106 FlgM-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCGGCGTTGTTGATGCAGATGGGAATA 58
O107 FlgM-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACGAAGTGCGATCAATACTCATGGTTTATT 58
O108 FlhB-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCACGTCATATCAGGCGGTCTGATAAGG 58
O109 FlhB-R GTTTCTTCCTGCAGCGGCCGCTACTAGTAGTTTTGTCGTCGCTCTCGTCAGACACGTC 58
O110 FlhDC-Uri-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTTGTATGTGCGTGTAGTGACGAGTACAG 58
O111 FlhDC-Total-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTCATTTTTGCTTGCTAGCGTACGGAAAA 58
O112 FlhDC(rbs)-R GTTTCTTCCTGCAGCGGCCGCTACTAGTATCCCACCCAGAATAACCAACTTTAT 54
O113 FlhDC(nu)-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGAATAACCAACTTTATTTTTATG 54
O114 FliA-PCR1-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCTGATTAACTGAGACTGACGGCAACGC 58
O115 FliA-PCR1-R CAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCCGCAGGGTTTTTAATCG 59
O116 FliA-PCR2-F GAAACGGATAATCATGCCGATAACTCATATAACGCAGGGCTG 42
O117 FliA-PCR2-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACCATTACACCTTCAGCGGTATAGAGTGAACTCACGATAAA 69
O118 FliC-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCAGCGATGAAATACTTGCCATGCGATT 58
O119 FliC-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGCGAGAGGCTGTTGGTATTAATGACTT 58
O120 FliE-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGTCGCATTCATCGCGCACCTCGTGGCTG 58
O121 FliE-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACTGGCTGATAACCCCTTCAATCCCCTGTA 58
O122 FliF-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGGCTGATAACCCCTTCAATCCCCTGT 58
O123 FliF-R GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGGCTGATAACCCCTTCAATCCCCTGT 58
O124 FliL-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGCGAGAGGCTGTTGGTATTAATGACT 58
O125 FliL-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACCAGCGATGAAATACTTGCCATGCGATTT 58
O126 Gene-EnvZ-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGGCGATTGCGCTTCTCGCCAC 53
O127 Gene-EnvZ-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTACCCTTCTTTTGTCGTGCCCTGCGCC 59
O128 Gene-FlgM-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTATTGATCGCACTTCGCCTC 53
O129 Gene-FlgM-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGTTACTCTGCAAGTCTTGCTGCGCT 59
O130 Gene-FlhC-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTGAAAAAAGCATTGTTCAGG 53
O131 Gene-FlhC-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAAACAGCCTGTACTCTCTGTTCATCC 59
O132 Gene-FlhD-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTGAAAC 53
O133 Gene-FlhD-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGGCCCTTTTCTTGCGCAGCGCTTCT 59
O134 Gene-FlhDCrbs-F GTTTCTTCGAATTCGCGGCCGCTTCTAGACCGCATAAAAATAAAGTTGGTTAT 53
O135 Gene-FliA-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATTCACTCTATACCGCTGAAG 53
O136 Gene-FliA-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTATAACTTACCCAGTTTAGTGCGTAAC 59
O137 Gene-FliArbs-F GTTTCTTCGAATTCGCGGCCGCTTCTAGTAATCATGCCGATAACTCATATAAC 53
O138 Gene-OmpR-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAAGAGAACTACAAGATTCTGG 53
O139 Gene-OmpR-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTATGCTTTAGAGCCGTCCGGTACAAAG 59
Retrieved from "http://2008.igem.org/Team:Paris/Notebook/Oligo"