Team:Paris/Notebook/Oligo

From 2008.igem.org

(Difference between revisions)
Line 11: Line 11:
| style="background: #ccffcc;" |O100
| style="background: #ccffcc;" |O100
| FlgA-F
| FlgA-F
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCATATCTCCTCCGCAGGTATCAAAAT
-
|  
+
| 58
|  
|  
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O101
| style="background: #ccffcc;" | O101
| FlgA-R
| FlgA-R
-
|  
+
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAACAGTATCGCGATGATCGCCACGCTACGT
-
|  
+
| 58
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O102
| style="background: #ccffcc;" | O102
| FlgB-F
| FlgB-F
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGACAGTATCGCGATGATCGCCACGCTACG
-
|  
+
| 58
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O103
| style="background: #ccffcc;" | O103
| FlgB-R
| FlgB-R
-
|  
+
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGCATATCTCCTCCGCAGGTATCAAAATT
-
|  
+
| 58
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O104
| style="background: #ccffcc;" | O104
| FlgK-F
| FlgK-F
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGCAGATGAAATCGATAAGCGACAAGG
-
|  
+
| 58
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O105
| style="background: #ccffcc;" | O105
| FlgK-R
| FlgK-R
-
|  
+
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAAACGCCGCCTGGGCCGCGTTCAGTCCGCT
-
|  
+
| 58
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O106
| style="background: #ccffcc;" | O106
| FlgM-F
| FlgM-F
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCGGCGTTGTTGATGCAGATGGGAATA
-
|  
+
| 58
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O107
| style="background: #ccffcc;" | O107
| FlgM-R
| FlgM-R
-
|  
+
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACGAAGTGCGATCAATACTCATGGTTTATT
-
|  
+
| 58
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O108
| style="background: #ccffcc;" | O108
| FlhB-F
| FlhB-F
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCACGTCATATCAGGCGGTCTGATAAGG
-
|  
+
| 58
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O109
| style="background: #ccffcc;" | O109
| FlhB-R
| FlhB-R
-
|  
+
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAGTTTTGTCGTCGCTCTCGTCAGACACGTC
-
|  
+
| 58
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O110
| style="background: #ccffcc;" | O110
| FlhDC-Uri-F
| FlhDC-Uri-F
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTTGTATGTGCGTGTAGTGACGAGTACAG
-
|  
+
| 58
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O111
| style="background: #ccffcc;" | O111
| FlhDC-Total-F
| FlhDC-Total-F
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTCATTTTTGCTTGCTAGCGTACGGAAAA
-
|  
+
| 58
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O112
| style="background: #ccffcc;" | O112
| FlhDC(rbs)-R
| FlhDC(rbs)-R
-
|  
+
| GTTTCTTCCTGCAGCGGCCGCTACTAGTATCCCACCCAGAATAACCAACTTTAT
-
|  
+
| 54
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O113
| style="background: #ccffcc;" | O113
| FlhDC(nu)-R
| FlhDC(nu)-R
-
|  
+
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGAATAACCAACTTTATTTTTATG
-
|  
+
| 54
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O114
| style="background: #ccffcc;" | O114
| FliA-PCR1-F
| FliA-PCR1-F
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCTGATTAACTGAGACTGACGGCAACGC
-
|  
+
| 58
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O115
| style="background: #ccffcc;" | O115
| FliA-PCR1-R
| FliA-PCR1-R
-
|  
+
| CAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCCGCAGGGTTTTTAATCG
-
|  
+
| 59
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O116
| style="background: #ccffcc;" | O116
| FliA-PCR2-F
| FliA-PCR2-F
-
|  
+
| GAAACGGATAATCATGCCGATAACTCATATAACGCAGGGCTG
-
|  
+
| 42
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O117
| style="background: #ccffcc;" | O117
| FliA-PCR2-R
| FliA-PCR2-R
-
|  
+
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACCATTACACCTTCAGCGGTATAGAGTGAACTCACGATAAA
-
|  
+
| 69
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O118
| style="background: #ccffcc;" | O118
| FliC-F
| FliC-F
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCAGCGATGAAATACTTGCCATGCGATT
-
|  
+
| 58
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O119
| style="background: #ccffcc;" | O119
| FliC-R
| FliC-R
-
|  
+
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGCGAGAGGCTGTTGGTATTAATGACTT
-
|  
+
| 58
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O120
| style="background: #ccffcc;" | O120
| FliE-F
| FliE-F
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGTCGCATTCATCGCGCACCTCGTGGCTG
-
|  
+
| 58
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O121
| style="background: #ccffcc;" | O121
| FliE-R
| FliE-R
-
|  
+
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACTGGCTGATAACCCCTTCAATCCCCTGTA
-
|  
+
| 58
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O122
| style="background: #ccffcc;" | O122
| FliF-F
| FliF-F
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGGCTGATAACCCCTTCAATCCCCTGT
-
|  
+
| 58
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O123
| style="background: #ccffcc;" | O123
| FliF-R
| FliF-R
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGGCTGATAACCCCTTCAATCCCCTGT
-
|  
+
| 58
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O124
| style="background: #ccffcc;" | O124
| FliL-F
| FliL-F
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGCGAGAGGCTGTTGGTATTAATGACT
-
|  
+
| 58
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O125
| style="background: #ccffcc;" | O125
| FliL-R
| FliL-R
-
|  
+
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACCAGCGATGAAATACTTGCCATGCGATTT
-
|  
+
| 58
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O126
| style="background: #ccffcc;" | O126
| Gene-EnvZ-F
| Gene-EnvZ-F
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGGCGATTGCGCTTCTCGCCAC
-
|  
+
| 53
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O127
| style="background: #ccffcc;" | O127
| Gene-EnvZ-R
| Gene-EnvZ-R
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTACCCTTCTTTTGTCGTGCCCTGCGCC
-
|  
+
| 59
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O128
| style="background: #ccffcc;" | O128
| Gene-FlgM-F
| Gene-FlgM-F
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTATTGATCGCACTTCGCCTC
-
|  
+
| 53
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O129
| style="background: #ccffcc;" | O129
| Gene-FlgM-R
| Gene-FlgM-R
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGTTACTCTGCAAGTCTTGCTGCGCT
-
|  
+
| 59
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O130
| style="background: #ccffcc;" | O130
| Gene-FlhC-F
| Gene-FlhC-F
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTGAAAAAAGCATTGTTCAGG
-
|  
+
| 53
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O131
| style="background: #ccffcc;" | O131
| Gene-FlhC-R
| Gene-FlhC-R
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAAACAGCCTGTACTCTCTGTTCATCC
-
|  
+
| 59
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O132
| style="background: #ccffcc;" | O132
| Gene-FlhD-F
| Gene-FlhD-F
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTGAAAC
-
|  
+
| 53
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O133
| style="background: #ccffcc;" | O133
| Gene-FlhD-R
| Gene-FlhD-R
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGGCCCTTTTCTTGCGCAGCGCTTCT
-
|  
+
| 59
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O134
| style="background: #ccffcc;" | O134
| Gene-FlhDCrbs-F
| Gene-FlhDCrbs-F
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGACCGCATAAAAATAAAGTTGGTTAT
-
|  
+
| 53
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O135
| style="background: #ccffcc;" | O135
| Gene-FliA-F
| Gene-FliA-F
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATTCACTCTATACCGCTGAAG
-
|  
+
| 53
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O136
| style="background: #ccffcc;" | O136
| Gene-FliA-R
| Gene-FliA-R
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTATAACTTACCCAGTTTAGTGCGTAAC
-
|  
+
| 59
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O137
| style="background: #ccffcc;" | O137
| Gene-FliArbs-F
| Gene-FliArbs-F
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTAATCATGCCGATAACTCATATAAC
-
|  
+
| 53
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O138
| style="background: #ccffcc;" | O138
| Gene-OmpR-F
| Gene-OmpR-F
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAAGAGAACTACAAGATTCTGG
-
|  
+
| 53
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | O139
| style="background: #ccffcc;" | O139
| Gene-OmpR-R
| Gene-OmpR-R
-
|  
+
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTATGCTTTAGAGCCGTCCGGTACAAAG
-
|  
+
| 59
|
|
|}
|}

Revision as of 11:32, 30 July 2008

Table of Oligos
Number Name Sequence Length Comments
O100 FlgA-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCATATCTCCTCCGCAGGTATCAAAAT 58
O101 FlgA-R GTTTCTTCCTGCAGCGGCCGCTACTAGTAACAGTATCGCGATGATCGCCACGCTACGT 58
O102 FlgB-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGACAGTATCGCGATGATCGCCACGCTACG 58
O103 FlgB-R GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGCATATCTCCTCCGCAGGTATCAAAATT 58
O104 FlgK-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGCAGATGAAATCGATAAGCGACAAGG 58
O105 FlgK-R GTTTCTTCCTGCAGCGGCCGCTACTAGTAAACGCCGCCTGGGCCGCGTTCAGTCCGCT 58
O106 FlgM-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCGGCGTTGTTGATGCAGATGGGAATA 58
O107 FlgM-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACGAAGTGCGATCAATACTCATGGTTTATT 58
O108 FlhB-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCACGTCATATCAGGCGGTCTGATAAGG 58
O109 FlhB-R GTTTCTTCCTGCAGCGGCCGCTACTAGTAGTTTTGTCGTCGCTCTCGTCAGACACGTC 58
O110 FlhDC-Uri-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTTGTATGTGCGTGTAGTGACGAGTACAG 58
O111 FlhDC-Total-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTCATTTTTGCTTGCTAGCGTACGGAAAA 58
O112 FlhDC(rbs)-R GTTTCTTCCTGCAGCGGCCGCTACTAGTATCCCACCCAGAATAACCAACTTTAT 54
O113 FlhDC(nu)-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGAATAACCAACTTTATTTTTATG 54
O114 FliA-PCR1-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCTGATTAACTGAGACTGACGGCAACGC 58
O115 FliA-PCR1-R CAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCCGCAGGGTTTTTAATCG 59
O116 FliA-PCR2-F GAAACGGATAATCATGCCGATAACTCATATAACGCAGGGCTG 42
O117 FliA-PCR2-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACCATTACACCTTCAGCGGTATAGAGTGAACTCACGATAAA 69
O118 FliC-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCAGCGATGAAATACTTGCCATGCGATT 58
O119 FliC-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGCGAGAGGCTGTTGGTATTAATGACTT 58
O120 FliE-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGTCGCATTCATCGCGCACCTCGTGGCTG 58
O121 FliE-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACTGGCTGATAACCCCTTCAATCCCCTGTA 58
O122 FliF-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGGCTGATAACCCCTTCAATCCCCTGT 58
O123 FliF-R GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGGCTGATAACCCCTTCAATCCCCTGT 58
O124 FliL-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGCGAGAGGCTGTTGGTATTAATGACT 58
O125 FliL-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACCAGCGATGAAATACTTGCCATGCGATTT 58
O126 Gene-EnvZ-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGGCGATTGCGCTTCTCGCCAC 53
O127 Gene-EnvZ-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTACCCTTCTTTTGTCGTGCCCTGCGCC 59
O128 Gene-FlgM-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTATTGATCGCACTTCGCCTC 53
O129 Gene-FlgM-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGTTACTCTGCAAGTCTTGCTGCGCT 59
O130 Gene-FlhC-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTGAAAAAAGCATTGTTCAGG 53
O131 Gene-FlhC-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAAACAGCCTGTACTCTCTGTTCATCC 59
O132 Gene-FlhD-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTGAAAC 53
O133 Gene-FlhD-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGGCCCTTTTCTTGCGCAGCGCTTCT 59
O134 Gene-FlhDCrbs-F GTTTCTTCGAATTCGCGGCCGCTTCTAGACCGCATAAAAATAAAGTTGGTTAT 53
O135 Gene-FliA-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATTCACTCTATACCGCTGAAG 53
O136 Gene-FliA-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTATAACTTACCCAGTTTAGTGCGTAAC 59
O137 Gene-FliArbs-F GTTTCTTCGAATTCGCGGCCGCTTCTAGTAATCATGCCGATAACTCATATAAC 53
O138 Gene-OmpR-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAAGAGAACTACAAGATTCTGG 53
O139 Gene-OmpR-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTATGCTTTAGAGCCGTCCGGTACAAAG 59
Retrieved from "http://2008.igem.org/Team:Paris/Notebook/Oligo"