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Team:Paris/Notebook/Oligo
From 2008.igem.org
(Difference between revisions)
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| - | | | + | |Don't amplify the both OmpR binding site |
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| - | | | + | |Amplify the natural rbs of FlhD |
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| style="background: #ccffcc;" | O113 | | style="background: #ccffcc;" | O113 | ||
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| - | | | + | |Don't amplify the natural rbs of FlhD (only promoter) |
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| style="background: #ccffcc;" | O114 | | style="background: #ccffcc;" | O114 | ||
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| - | | | + | |Don't amplify the natural rbs of FlhD |
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| style="background: #ccffcc;" | O133 | | style="background: #ccffcc;" | O133 | ||
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| - | | | + | |Amplify the natural rbs of FlhD so that we have FlhD and FlhC with both their natural rbs |
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| style="background: #ccffcc;" | O135 | | style="background: #ccffcc;" | O135 | ||
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| - | | | + | |Don't Amplify the natural rbs of FliA |
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| style="background: #ccffcc;" | O136 | | style="background: #ccffcc;" | O136 | ||
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| - | | | + | |Amplify the natural rbs of FliA |
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| style="background: #ccffcc;" | O138 | | style="background: #ccffcc;" | O138 | ||
Revision as of 11:41, 30 July 2008
| Table of Oligos | ||||||
|---|---|---|---|---|---|---|
| Number | Name | Sequence | Length | Tm(°C) | Comments | |
| O100 | FlgA-F | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCATATCTCCTCCGCAGGTATCAAAAT | 58 | |||
| O101 | FlgA-R | GTTTCTTCCTGCAGCGGCCGCTACTAGTAACAGTATCGCGATGATCGCCACGCTACGT | 58 | |||
| O102 | FlgB-F | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGACAGTATCGCGATGATCGCCACGCTACG | 58 | |||
| O103 | FlgB-R | GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGCATATCTCCTCCGCAGGTATCAAAATT | 58 | |||
| O104 | FlgK-F | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGCAGATGAAATCGATAAGCGACAAGG | 58 | |||
| O105 | FlgK-R | GTTTCTTCCTGCAGCGGCCGCTACTAGTAAACGCCGCCTGGGCCGCGTTCAGTCCGCT | 58 | |||
| O106 | FlgM-F | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCGGCGTTGTTGATGCAGATGGGAATA | 58 | |||
| O107 | FlgM-R | GTTTCTTCCTGCAGCGGCCGCTACTAGTACGAAGTGCGATCAATACTCATGGTTTATT | 58 | |||
| O108 | FlhB-F | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCACGTCATATCAGGCGGTCTGATAAGG | 58 | |||
| O109 | FlhB-R | GTTTCTTCCTGCAGCGGCCGCTACTAGTAGTTTTGTCGTCGCTCTCGTCAGACACGTC | 58 | |||
| O110 | FlhDC-Uri-F | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTTGTATGTGCGTGTAGTGACGAGTACAG | 58 | Don't amplify the both OmpR binding site | ||
| O111 | FlhDC-Total-F | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTCATTTTTGCTTGCTAGCGTACGGAAAA | 58 | Amplify the both OmpR binding site | ||
| O112 | FlhDC(rbs)-R | GTTTCTTCCTGCAGCGGCCGCTACTAGTATCCCACCCAGAATAACCAACTTTAT | 54 | Amplify the natural rbs of FlhD | ||
| O113 | FlhDC(nu)-R | GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGAATAACCAACTTTATTTTTATG | 54 | Don't amplify the natural rbs of FlhD (only promoter) | ||
| O114 | FliA-PCR1-F | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCTGATTAACTGAGACTGACGGCAACGC | 58 | |||
| O115 | FliA-PCR1-R | CAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCCGCAGGGTTTTTAATCG | 59 | |||
| O116 | FliA-PCR2-F | GAAACGGATAATCATGCCGATAACTCATATAACGCAGGGCTG | 42 | |||
| O117 | FliA-PCR2-R | GTTTCTTCCTGCAGCGGCCGCTACTAGTACCATTACACCTTCAGCGGTATAGAGTGAACTCACGATAAA | 69 | |||
| O118 | FliC-F | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCAGCGATGAAATACTTGCCATGCGATT | 58 | |||
| O119 | FliC-R | GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGCGAGAGGCTGTTGGTATTAATGACTT | 58 | |||
| O120 | FliE-F | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGTCGCATTCATCGCGCACCTCGTGGCTG | 58 | |||
| O121 | FliE-R | GTTTCTTCCTGCAGCGGCCGCTACTAGTACTGGCTGATAACCCCTTCAATCCCCTGTA | 58 | |||
| O122 | FliF-F | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGGCTGATAACCCCTTCAATCCCCTGT | 58 | |||
| O123 | FliF-R | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGGCTGATAACCCCTTCAATCCCCTGT | 58 | |||
| O124 | FliL-F | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGCGAGAGGCTGTTGGTATTAATGACT | 58 | |||
| O125 | FliL-R | GTTTCTTCCTGCAGCGGCCGCTACTAGTACCAGCGATGAAATACTTGCCATGCGATTT | 58 | |||
| O126 | Gene-EnvZ-F | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGGCGATTGCGCTTCTCGCCAC | 53 | |||
| O127 | Gene-EnvZ-R | GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTACCCTTCTTTTGTCGTGCCCTGCGCC | 59 | |||
| O128 | Gene-FlgM-F | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTATTGATCGCACTTCGCCTC | 53 | |||
| O129 | Gene-FlgM-R | GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGTTACTCTGCAAGTCTTGCTGCGCT | 59 | |||
| O130 | Gene-FlhC-F | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTGAAAAAAGCATTGTTCAGG | 53 | |||
| O131 | Gene-FlhC-R | GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAAACAGCCTGTACTCTCTGTTCATCC | 59 | |||
| O132 | Gene-FlhD-F | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTGAAAC | 53 | Don't amplify the natural rbs of FlhD | ||
| O133 | Gene-FlhD-R | GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGGCCCTTTTCTTGCGCAGCGCTTCT | 59 | |||
| O134 | Gene-FlhDCrbs-F | GTTTCTTCGAATTCGCGGCCGCTTCTAGACCGCATAAAAATAAAGTTGGTTAT | 53 | Amplify the natural rbs of FlhD so that we have FlhD and FlhC with both their natural rbs | ||
| O135 | Gene-FliA-F | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATTCACTCTATACCGCTGAAG | 53 | Don't Amplify the natural rbs of FliA | ||
| O136 | Gene-FliA-R | GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTATAACTTACCCAGTTTAGTGCGTAAC | 59 | |||
| O137 | Gene-FliArbs-F | GTTTCTTCGAATTCGCGGCCGCTTCTAGTAATCATGCCGATAACTCATATAAC | 53 | Amplify the natural rbs of FliA | ||
| O138 | Gene-OmpR-F | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAAGAGAACTACAAGATTCTGG | 53 | |||
| O139 | Gene-OmpR-R | GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTATGCTTTAGAGCCGTCCGGTACAAAG | 59 | |||
