Team:Princeton/ListofPlasmidDesigns

From 2008.igem.org

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[https://2008.igem.org/Media:pLV-Ubc-rtTA-2A-LacIKrab-IRES-Puro.pdf pLV-Ubc-rtTA-2A-LacIKrab-IRES-Puro]

Revision as of 03:05, 30 October 2008

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PRINCETON IGEM 2008

Home Project Overview Project Details Experiments Results Notebook
Parts Submitted to the Registry Modeling The Team Gallery




The highlight code is as follows:

style="background:yellow; color:black" | yellow: overlap of primers with the DNA
style="background:green; color:black" |dark green: restriction site
style="background:magenta; color:black" |pink: "junk" sequence to allow restriction enzymes to cut near the end of a strand.
style="background:cyan; color:black" |light blue: kozak sequence.
style="background:teal; color:black" |dark blue: stop codon.
style="background:gray; color:black" |gray: a 2A sequence (the amino acid sequence is cut by the cell)
style="background:red; color:black" |red: either a linker to fuse two proteins (where indicated) or a sequence with a restriction site removed by changing a base pair with the primer.
pLV-Hef1aLacO-MashI-2A-ChAT-Ubc-Hyg

pLV-Hef1aLacO-MashI-2A-Nurr1-Ubc-Hyg

pLV-Hef1a-LacO-MashI-Cerulean-Ubc-Hyg

pLV-Hef1a-LacO-MashI-EGFP-Ubc-Hyg

pLV-Hef1a-LacO-MashI-Ubc-Hyg

pLV-Hef1a-LacO-MashI-Ubc-Hyg2

pLV-Hef1aLacO-Ngn1-Ubc-Hyg

pLV-Hef1aLacO-Ngn2-Ubc-Hyg

pLV-Hef1aLacO-Nurr1-Ubc-Hyg

pLV-Hef1aLacO-Tlx3-Ubc-Hyg

pLV-pLux-ChAT-Ubc-Neo

pLV-TRE-Cav3.1

pLV-TRE-Cav3.1-Ubc-Bleo

pLV-TRE-D5R-Ubc-Bleo

pLV-TRE-GLRA1-2A-gephyrin-Ubc-Neo

pLV-TRE-GLRA1-Ubc-Neo

pLV-TRE-GLYT2-2A-Viaat-Ubc-Bla

pLV-TRE-HCN2-Ubc-Bla

pLV-TRE-Lbx1-2A-GLRA1-2A-D5R-Ubc-Bla

pLV-TRE-Lbx1-Ubc-Puro

pLV-TRE-MashI-IRES2-mKate

pLV-TRE-Neuregulin1-Ubc-Neo

pLV-TRE-Ngn1-EYFP-Ubc-Bla

pLV-Ubc-rtTA-2A-LacIKrab-2A-LuxR-IRES-Puro

pLV-Ubc-rtTA-2A-LacIKrab-IRES-Puro