Team:Rice University/Notebook/11 July 2008

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(Difference between revisions)
 
Line 8: Line 8:
****1uL BSA
****1uL BSA
****22uL dH2O
****22uL dH2O
 +
***DCC
 +
***Ligation Mix
 +
**** 10uL DCCed DNA
 +
****1uL T4
 +
****2uL Buffer
 +
****.5uL Vector
 +
****7.5 dH2O
 +
*Taylor Stevenson
*Taylor Stevenson
**Prepare 5mL LB O/N of VCS257 (37*C) and VCS257 lambda lysogens (30*C) for preparation of electro-competent cells  
**Prepare 5mL LB O/N of VCS257 (37*C) and VCS257 lambda lysogens (30*C) for preparation of electro-competent cells  

Latest revision as of 18:05, 11 July 2008

  • David Ouyang
    • Digest and Ligate Putative Amber Suppressors
      • Digest Mix
        • 1uL EcoRI
        • 1uL PstI
        • 5uL NEB3
        • 20uL Dilute DNA
        • 1uL BSA
        • 22uL dH2O
      • DCC
      • Ligation Mix
        • 10uL DCCed DNA
        • 1uL T4
        • 2uL Buffer
        • .5uL Vector
        • 7.5 dH2O
  • Taylor Stevenson
    • Prepare 5mL LB O/N of VCS257 (37*C) and VCS257 lambda lysogens (30*C) for preparation of electro-competent cells





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