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Team:The University of Alberta/28 July 2008
From 2008.igem.org
(Difference between revisions)
(New page: == Today in Lab== 1. Religate TetR to RBS (assuming there are digests still available)<br> 2. Resequence the TetR and RBS stocks<br> 3. Obtain p0380 vector and begin site directed mutagene...) |
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| - | == | + | ==To Do== |
1. Religate TetR to RBS (assuming there are digests still available)<br> | 1. Religate TetR to RBS (assuming there are digests still available)<br> | ||
2. Resequence the TetR and RBS stocks<br> | 2. Resequence the TetR and RBS stocks<br> | ||
| Line 6: | Line 6: | ||
5. Harvest seeds<br> | 5. Harvest seeds<br> | ||
6. Sequence J6+tryp transformants<br> | 6. Sequence J6+tryp transformants<br> | ||
| + | |||
| + | ==Today== | ||
| + | *The transformants of Tryp in J6 done on Friday turned out well. Picked 3 colonies and did: | ||
| + | **colony PCR | ||
| + | **streaking for SCIs | ||
| + | **set up O/Ns for minipreps tomorrow | ||
Revision as of 17:06, 28 July 2008
To Do
1. Religate TetR to RBS (assuming there are digests still available)
2. Resequence the TetR and RBS stocks
3. Obtain p0380 vector and begin site directed mutagenesis
4. Clean pots and plant more plants
5. Harvest seeds
6. Sequence J6+tryp transformants
Today
- The transformants of Tryp in J6 done on Friday turned out well. Picked 3 colonies and did:
- colony PCR
- streaking for SCIs
- set up O/Ns for minipreps tomorrow
