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Team:UNIPV-Pavia/Notebook/Week4
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(Difference between revisions)
| Line 38: | Line 38: | ||
'''06/09/08''' | '''06/09/08''' | ||
<br> | <br> | ||
| - | *We prepared | + | *We prepared 4 glycerol stocks taking 800 µl from 9 ml cultures containing: |
{|cellpadding="20" | {|cellpadding="20" | ||
| - | |BBa_B0030 ( | + | |BBa_B0030 (one of the two cultures) |
|BBa_R0051 | |BBa_R0051 | ||
|BBa_E0240 | |BBa_E0240 | ||
|- | |- | ||
| - | |||
|BBa_J23100-'''BBa_B0030''' | |BBa_J23100-'''BBa_B0030''' | ||
|} | |} | ||
| - | *Miniprep for | + | *Miniprep for the 5 cultures. |
| - | *We performed | + | *We performed digestion protocol to open plasmids: |
{|cellpadding="20" | {|cellpadding="20" | ||
|BBa_B0030 (1st culture) (E-X) | |BBa_B0030 (1st culture) (E-X) | ||
Revision as of 10:15, 6 July 2008
 Home
|  The Team
|  The Project
|  Biological Safety
|  Parts Submitted to the Registry
|
|---|---|---|---|---|
 Dry Lab
|  Wet Lab
|  Modeling
|  Protocols
|  Activity Notebook
|
Notebook
| Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 |
|---|
Week 4: 06/09/08 - 06/13/08
06/09/08
- We prepared 4 glycerol stocks taking 800 µl from 9 ml cultures containing:
| BBa_B0030 (one of the two cultures) | BBa_R0051 | BBa_E0240 |
| BBa_J23100-BBa_B0030 |
- Miniprep for the 5 cultures.
- We performed digestion protocol to open plasmids:
| BBa_B0030 (1st culture) (E-X) | BBa_R0051 (S-P) | BBa_E0240 (E-X) |
| BBa_B0030 (2nd culture) (S-P) |
- We performed PCR on extracted BBa_J23100-BBa_B0030.
06/10/08
