Team:UNIPV-Pavia/Notebook/Week4

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*We performed PCR on extracted BBa_J23100-'''BBa_B0030'''.
*We performed PCR on extracted BBa_J23100-'''BBa_B0030'''.
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*We performed electrophoresis on PCR result to check for contaminating plasmids: the plasmid was correct.
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Revision as of 10:22, 6 July 2008


Home.jpg Home Unipv logo.jpg The Team And.jpg The Project Safety.jpg Biological Safety Dna.png Parts Submitted to the Registry
Laptop.jpg Dry Lab Pipette.jpg Wet Lab Math.gif Modeling Note.jpg Protocols Notebook.gif Activity Notebook



Notebook



Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Week 7



Week 4: 06/09/08 - 06/13/08

06/09/08

  • We prepared 4 glycerol stocks taking 800 µl from 9 ml cultures containing:
BBa_B0030 (one of the two cultures) BBa_R0051 BBa_E0240
BBa_J23100-BBa_B0030
  • Miniprep for the 5 cultures.
  • We performed digestion protocol to open plasmids:
BBa_B0030 (1st culture) (E-X) BBa_R0051 (S-P) BBa_E0240 (E-X)
BBa_B0030 (2nd culture) (S-P)
  • We performed PCR on extracted BBa_J23100-BBa_B0030.
  • We performed electrophoresis on PCR result to check for contaminating plasmids: the plasmid was correct.
PCR results for BBa_J23100-BBa_B0030 ligation: plasmid length is correct



06/10/08

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