Team:UNIPV-Pavia/Notebook/Week4

From 2008.igem.org

(Difference between revisions)
Line 38: Line 38:
'''06/09/08'''
'''06/09/08'''
<br>
<br>
-
*We prepared 6 glycerol stocks taking 800 µl from 9 ml cultures containing:
+
*We prepared 5 glycerol stocks taking 800 µl from 9 ml cultures containing:
{|cellpadding="20"
{|cellpadding="20"
Line 47: Line 47:
|BBa_B0030 (2nd culture)
|BBa_B0030 (2nd culture)
|BBa_J23100-'''BBa_B0030'''
|BBa_J23100-'''BBa_B0030'''
 +
|}
 +
 +
*Miniprep for these 5 cultures.
 +
 +
*We performed excided fragments digestion protocol to open plasmids:
 +
{|cellpadding="20"
 +
|BBa_B0030 (1st culture) (E-X)
 +
|BBa_R0051 (S-P)
 +
|BBa_E0240 (E-X)
 +
|-
 +
|BBa_B0030 (2nd culture) (S-P)
|}
|}
-
*Miniprep for our BBa_B0030 (1st culture), BBa_B0030 (2nd culture)
 
<br><br>
<br><br>
-
'''06/09/08'''
+
'''06/10/08'''
<br>
<br>

Revision as of 09:46, 9 June 2008


Home.jpg Home Unipv logo.jpg The Team And.jpg The Project Safety.jpg Biological Safety Dna.png Parts Submitted to the Registry
Laptop.jpg Dry Lab Pipette.jpg Wet Lab Math.gif Modeling Note.jpg Protocols Notebook.gif Activity Notebook



Notebook



Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Week 7



Week 4: 06/09/08 - 06/13/08

06/09/08

  • We prepared 5 glycerol stocks taking 800 µl from 9 ml cultures containing:
BBa_B0030 (1st culture) BBa_R0051 BBa_E0240
BBa_B0030 (2nd culture) BBa_J23100-BBa_B0030
  • Miniprep for these 5 cultures.
  • We performed excided fragments digestion protocol to open plasmids:
BBa_B0030 (1st culture) (E-X) BBa_R0051 (S-P) BBa_E0240 (E-X)
BBa_B0030 (2nd culture) (S-P)




06/10/08

Retrieved from "http://2008.igem.org/Team:UNIPV-Pavia/Notebook/Week4"