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Team:UNIPV-Pavia/Notebook/Week6
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Notebook
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Week 5: 06/23/08 - 06/20/08
06/23/08
- Colony PCR for BBa_B0030-BBa_C0061 and BBa_B0030 (no Ant.Phosph.)-BBa_C0061: 10 colonies for every plate.
- We ran PCR results: some colonies showed both ligated plasmid (heavier band) and false positive plasmid, because it was difficult to pick up single colonies from bacteria carpet. Ligation with Antarctic Phosphatase showed non-pure colonies and 3 false positives. Ligation without Antarctic Phosphatase showed 3 non-pure colonies and 7 pure colonies.
- We decided to avoid Antarctic Phosphatase treatment in next ligations to save time.
- We choose 4th and 7th colonies to grow 9 ml cultures overnight.
- We also infected 9 ml LB + Amp with30
- We transformed the remaining ligations:
- BBa_B0030-BBa_E0040
- BBa_B0030-BBa_E1010
- BBa_B0030-BBa_C0051
