Team:UNIPV-Pavia/Notebook/Week7

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*Colony PCR for '''BBa_J23100'''-BBa_E0240 and '''BBa_B0030'''-BBa_C0061: 10 colonies for every plate. Gel showed many working colonies: we chose first colonies for the two ligations.
*Colony PCR for '''BBa_J23100'''-BBa_E0240 and '''BBa_B0030'''-BBa_C0061: 10 colonies for every plate. Gel showed many working colonies: we chose first colonies for the two ligations.
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|[[Image:pv_pcr_01_08.jpg|thumb|300px|left|Colony PCR for '''BBa_J23100'''-BBa_E0240, '''BBa_B0030'''-BBa_E0061: Marker and 10 colonies for each ligation]]
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*We infected 9 ml LB + Amp with 30 µl of:
*We infected 9 ml LB + Amp with 30 µl of:

Revision as of 15:36, 13 July 2008


Home.jpg Home Unipv logo.jpg The Team And.jpg The Project Safety.jpg Biological Safety Dna.png Parts Submitted to the Registry
Laptop.jpg Dry Lab Pipette.jpg Wet Lab Math.gif Modeling Note.jpg Protocols Notebook.gif Activity Notebook



Notebook



Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Week 7



Week 5: 06/23/08 - 06/27/08

06/30/08

  • We received sequencing results for BBa_B0030-BBa_C0061 (4th colony and 7th colony): sequences were correct! We decided to keep the 4th colony.
  • Colony PCR for BBa_J23100-BBa_E0240 and BBa_B0030-BBa_C0061: 10 colonies for every plate. Gel showed many working colonies: we chose first colonies for the two ligations.
Colony PCR for BBa_J23100-BBa_E0240, BBa_B0030-BBa_E0061: Marker and 10 colonies for each ligation
  • We infected 9 ml LB + Amp with 30 µl of:
BBa_C0012 BBa_B1006 BBa_R0051-BBa_B0030 BBa_B0030-BBa_C0061 (4)
BBa_C0062 BBa_J23100-BBa_E0240 (1) BBa_B0030-BBa_C0078 (8) BBa_J23100-BBa_B0030
BBa_C0040 BBa_I15010



07/1/08