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Team:UNIPV-Pavia/Notebook/Week7
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*We received sequencing results for '''BBa_B0030'''-BBa_C0061 (4th colony and 7th colony): sequences were correct! We decided to keep the 4th colony. | *We received sequencing results for '''BBa_B0030'''-BBa_C0061 (4th colony and 7th colony): sequences were correct! We decided to keep the 4th colony. | ||
| - | * | + | *Colony PCR for '''BBa_J23100'''-BBa_E0240 and '''BBa_B0030'''-BBa_C0061: 10 colonies for every plate. Gel showed many working colonies: we chose first colonies for the two ligations. |
| - | * | + | *We infected 9 ml LB + Amp with 30 µl of: |
| + | {|cellpadding="20px" | ||
| + | |BBa_C0012 | ||
| + | |BBa_B1006 | ||
| + | |BBa_C0040 | ||
| + | |'''BBa_R0051'''-BBa_B0030 | ||
| + | |'''BBa_B0030'''-BBa_C0061 (4) | ||
| + | |- | ||
| + | |BBa_C0062 | ||
| + | |BBa_I15010 | ||
| + | |'''BBa_J23100'''-BBa_E0240 (1) | ||
| + | |'''BBa_B0030'''-BBa_C0078 (8) | ||
| + | |BBa_J23100-'''BBa_B0030''' | ||
| + | |} | ||
<br><br> | <br><br> | ||
'''07/1/08''' | '''07/1/08''' | ||
<br> | <br> | ||
Revision as of 15:04, 13 July 2008
 Home
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|  The Project
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|  Parts Submitted to the Registry
|
|---|---|---|---|---|
 Dry Lab
|  Wet Lab
|  Modeling
|  Protocols
|  Activity Notebook
|
Notebook
| Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 |
|---|
Week 5: 06/23/08 - 06/27/08
06/30/08
- We received sequencing results for BBa_B0030-BBa_C0061 (4th colony and 7th colony): sequences were correct! We decided to keep the 4th colony.
- Colony PCR for BBa_J23100-BBa_E0240 and BBa_B0030-BBa_C0061: 10 colonies for every plate. Gel showed many working colonies: we chose first colonies for the two ligations.
- We infected 9 ml LB + Amp with 30 µl of:
| BBa_C0012 | BBa_B1006 | BBa_C0040 | BBa_R0051-BBa_B0030 | BBa_B0030-BBa_C0061 (4) |
| BBa_C0062 | BBa_I15010 | BBa_J23100-BBa_E0240 (1) | BBa_B0030-BBa_C0078 (8) | BBa_J23100-BBa_B0030 |
07/1/08
