Team:UNIPV-Pavia/Notebook/Week7

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Notebook



Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Week 7



Week 7: 06/23/08 - 06/27/08

06/30/08

  • We received sequencing results for BBa_B0030-BBa_C0061 (4th colony and 7th colony): sequences were correct! We decided to keep the 4th colony.
  • Colony PCR for BBa_J23100-BBa_E0240 and BBa_B0030-BBa_C0061: 10 colonies for every plate. Gel showed many working colonies: we chose first colonies for the two ligations.
Colony PCR for BBa_J23100-BBa_E0240, BBa_B0030-BBa_E0061: Marker and 10 colonies for each ligation
  • We infected 9 ml LB + Amp with 30 µl of:
BBa_C0012 BBa_B1006 BBa_R0051-BBa_B0030 BBa_B0030-BBa_C0061 (4)
BBa_C0062 BBa_J23100-BBa_E0240 (1) BBa_B0030-BBa_C0078 (8) BBa_J23100-BBa_B0030
BBa_C0040 BBa_I15010



07/1/08