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Team:University of Chicago/Notebook/Norayucel
From 2008.igem.org
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==July 18, 2008== | ==July 18, 2008== | ||
| - | 1. Diluted 1ng/microliter pGreen plasmid to 10pg/microliter with 100X dilution | + | 1. Diluted 1ng/microliter pGreen plasmid to 10pg/microliter with 100X dilution<br> |
| - | 2. 1microliter of plasmid to 50 microliters competent cells | + | 2. 1microliter of plasmid to 50 microliters competent cells<br> |
| - | 3. Will grow up 50microliters of TOP10 untransformed as a control | + | 3. Will grow up 50microliters of TOP10 untransformed as a control<br> |
| - | 4. Put on ice at 2:03 | + | 4. Put on ice at 2:03<br> |
| - | 5. Took off ice at 2:34 | + | 5. Took off ice at 2:34<br> |
| - | 6. Incubated for 1minute at 42C | + | 6. Incubated for 1minute at 42C<br> |
| - | 7. Added 250microliters SOC media (prepared at 1:30) | + | 7. Added 250microliters SOC media (prepared at 1:30)<br> |
| - | 8. Start incubated at 37C at 2:36 | + | 8. Start incubated at 37C at 2:36<br> |
| - | 9. Take out at 3:40pm | + | 9. Take out at 3:40pm<br> |
| - | 10. Streaked 20microliters onto Amp. Plates | + | 10. Streaked 20microliters onto Amp. Plates<br> |
| - | 11. Dan will come in Saturday morning to pick up the plates and count colonies. | + | 11. Dan will come in Saturday morning to pick up the plates and count colonies.<br> |
Revision as of 21:24, 18 July 2008
July 18, 2008
1. Diluted 1ng/microliter pGreen plasmid to 10pg/microliter with 100X dilution
2. 1microliter of plasmid to 50 microliters competent cells
3. Will grow up 50microliters of TOP10 untransformed as a control
4. Put on ice at 2:03
5. Took off ice at 2:34
6. Incubated for 1minute at 42C
7. Added 250microliters SOC media (prepared at 1:30)
8. Start incubated at 37C at 2:36
9. Take out at 3:40pm
10. Streaked 20microliters onto Amp. Plates
11. Dan will come in Saturday morning to pick up the plates and count colonies.
