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Team:University of Chicago/Papers
From 2008.igem.org
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Please post links to relevant papers here with brief descriptions. Sign your name in case we have questions. I'll start us off with everything I've gotten by email so far.
Contents |
Expression
Patent: Method of producing bioadhesive protein. Making mussel proteins since 1990! ---Steve Kron
Patent: Bioadhesive precursor protein expression vectors. I swear these people just photocopied their application and mailed it out again. ---Steve Kron
Patent: Production of bioadhesive precursor protein analogs by genetically engineered organisms. New pictures this time. ---Steve Kron
Secretion
An E. coli Expression System for the Extracellular Secretion of Barley α-Amylase (pdf). A possible secretion pathway if we choose E. coli for our system. ---Nora Yucel
Type III protein secretion systems in Bacterial Pathogens of Plants and Animals (pdf). A nice review of natural type III secretion systems we can take advantage of.. ---Nora Yucel
http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=2074921&blobtype=pdf Cloning and Transfer of the Salmonella Pathogenicity Island 2 Type III Secretion System for Studies of a Range of Gram-Negative Genera (pdf). Secretion pathway on a stick, stick fed to bacteria. ---NY
Mgfp-5
Recombinant mussel adhesive protein Mgfp-5 as cell adhesion biomaterial. Modified Mgfp-5 is better than Cell-Tak or poly-L-lysine; unmodified is about the same as Cell-Tak. It survives harsher washes on glass than BSA or Cell-Tak. ---Steve Kron
Practical recombinant hybrid mussel bioadhesive fp-151. A recombinant fp-1/fp-5 fusion protein works in cell bioadhesion assays. 1g/L production and soluble. ---Steve Kron
Other
2007 Ideas. Great big honking list of references. ---Thomas Graham
"Quorum Sensing in E.Coli" Bioluminescence Activation via Q.S. -Rob M
