Team:University of Ottawa/18 July 2008

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(New page: __TOC__ ==Today in the Lab== '''Matt''' :'''PCR Amplification''' ::<li> A PCR Amplification was performed on 600/1 plasmids with integration primers so that yeast integration can be perfor...)
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::<li> The transformation was a success with both plates streaked growing numerous colonies.  
::<li> The transformation was a success with both plates streaked growing numerous colonies.  
::<li> For miniprep on Monday, I think I will choose 5 colonies per plate.
::<li> For miniprep on Monday, I think I will choose 5 colonies per plate.
 +
::<li> An inoculation will be performed today into liquid media so that a miniprep can be performed.

Revision as of 18:41, 18 July 2008

Contents

Today in the Lab

Matt

PCR Amplification
  • A PCR Amplification was performed on 600/1 plasmids with integration primers so that yeast integration can be performed next week. Corey suggested it is better to make new stock of this PCR amplification product for integration.
  • Transformation
  • The transformation was a success with both plates streaked growing numerous colonies.
  • For miniprep on Monday, I think I will choose 5 colonies per plate.
  • An inoculation will be performed today into liquid media so that a miniprep can be performed.