Team:University of Washington

From 2008.igem.org

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!align="center"; style="border-color:#6b0c6a;background-color:#500050"|[[Team:University_of_Washington|Home]]
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!align="center"; style="border-color:#6b0c6a"|[[Team:University_of_Washington/Team|The Team]]
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!align="center"; style="border-color:#6b0c6a"|[[Team:University_of_Washington/Project|The Project]]
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<h1> Abstract </h1>
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<h5>Transferring novel abilities into eukaryotes has many potential applications. Our project attempts to control transfer of genetic material across phylogenetic domains. We attempt to direct the prokaryote <i>Escherichia coli</i> (domain Bacteria) to transfer DNA encoding potentially useful traits from to the yeast <i>Saccharomyces cervisiae</i> (domain Fungi). The design utilizes standard engineering and synthetic biology techniques to modularize this process, in order to enable usage across varying organisms and conditions. To achieve control over our system, bacteria transfer DNA via conjugation only if certain conditions are met. In our design, <i>E. coli</i> transfers the genes to metabolize lactose in <i>S. cerevisiae</i>, but only where lactose is prevalent, glucose is minimal, and yeast proximity is sensed via a yeast-produced signaling molecule. It therefore provides a means for conditional, not constitutive, gene transfer between diverse organisms. Applications might include the production of transgenic plants and animals, clinical gene delivery, and interacting multiple-organism systems. </h5>
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== Abstract ==
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Our project seeks to control transfer of genetic material across phylogenetic domains. Specifically, we will attempt to transfer conjugative plasmids from the prokaryote ''Escherichia coli'' (domain Bacteria) to the yeast ''Saccharomyces cervisiae'' (domain Fungi). However, the design is intended to promote modular substituion to enable usage across different organisms and under various conditions. In our particular design, the bacteria will express conjugative machinery and transfer genetic material in the presence of certain molecular signals, the absence of others, and only when yeast is present.
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The conjugative plasmids transferred from ''E. coli'' to ''S. cervisiae'' will confer the ability for yeast to perform some adaptive function; in our case, it will be the ability to digest lactose. We will induce the production of conjugation machinery in ''E. coli'' under conditions where lactose is prevalent, glucose is minimal, and yeast proximity is sensed via a yeast-produced signaling molecule. The use of mutualistic interactions under conditions of selective pressure will assist in maintaining long-term functionality of genetic circuitry. Future directions for this project might include clinical gene delivery, more reliable transformation of other eukartyoes, and interacting multiple-organism systems.
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[[Image:UW Team.jpg|thumb|500px|center|'''Back row, left to right:''' Param, Jeff, Alec, Bryan, Tyler '''Front:''' Faifan, Scott ]]
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<!--- The Mission, Experiments --->
<!--- The Mission, Experiments --->

Latest revision as of 23:46, 27 October 2008

WashingtonColorSeal-21-clip.gif Home The Team The Project Modeling Notebook Protocols Parts Submitted
to the Registry
Measurement Kit SeToB Safety

Abstract

Transferring novel abilities into eukaryotes has many potential applications. Our project attempts to control transfer of genetic material across phylogenetic domains. We attempt to direct the prokaryote Escherichia coli (domain Bacteria) to transfer DNA encoding potentially useful traits from to the yeast Saccharomyces cervisiae (domain Fungi). The design utilizes standard engineering and synthetic biology techniques to modularize this process, in order to enable usage across varying organisms and conditions. To achieve control over our system, bacteria transfer DNA via conjugation only if certain conditions are met. In our design, E. coli transfers the genes to metabolize lactose in S. cerevisiae, but only where lactose is prevalent, glucose is minimal, and yeast proximity is sensed via a yeast-produced signaling molecule. It therefore provides a means for conditional, not constitutive, gene transfer between diverse organisms. Applications might include the production of transgenic plants and animals, clinical gene delivery, and interacting multiple-organism systems.
Vectorjector.jpg