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Team:Valencia/Notebook/August
From 2008.igem.org
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[[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br> | [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br> | ||
Galactose activation at two, three and four hours. <br> | Galactose activation at two, three and four hours. <br> | ||
| - | | + | O.D. measured at each time. <br> |
===August 7th=== | ===August 7th=== | ||
-We added palmitic acid to see if it had any effect, especially in UCP+ strain. Experiment: <br> | -We added palmitic acid to see if it had any effect, especially in UCP+ strain. Experiment: <br> | ||
[[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br> | [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br> | ||
| - | Strains UCP-, 76Δ and UCP+ with galactose induction and palmitic acid at 3 hours after inoculating. | + | Strains UCP-, 76Δ and UCP+ with galactose induction and palmitic acid at 3 hours after inoculating.<br> |
| - | Strain UCP+ just with galactose induction. | + | Strain UCP+ just with galactose induction.<br> |
| + | |||
| + | ===August 8th=== | ||
| + | -We prepared lab stuff and decided the following experiment we would carry out. | ||
| + | |||
| + | ===August 9th=== | ||
| + | -We carried out the experiment changing some variables: | ||
| + | [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br> | ||
| + | Four strains. <br> | ||
| + | Inoculation at O.D. around 0.2 <br> | ||
| + | Galactose induction 5 hours after inoculating. <br> | ||
| + | |||
| + | |||
<html> | <html> | ||
Revision as of 19:44, 12 August 2008
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August 1st
- We carried out the experiment with Medium YPKAc. We were just characterizing yeast growth in this medium. In this medium containing acetate, yeast is supposed to only breathe rather than fermenting.
August 5th
- Experiment carried out:
SP medium.
Four strains.
No galactose activation.
August 6th
-Trying to find out the best moment for galactose activation....
SP medium.
Galactose activation at two, three and four hours.
O.D. measured at each time.
August 7th
-We added palmitic acid to see if it had any effect, especially in UCP+ strain. Experiment:
SP medium.
Strains UCP-, 76Δ and UCP+ with galactose induction and palmitic acid at 3 hours after inoculating.
Strain UCP+ just with galactose induction.
August 8th
-We prepared lab stuff and decided the following experiment we would carry out.
August 9th
-We carried out the experiment changing some variables:
SP medium.
Four strains.
Inoculation at O.D. around 0.2
Galactose induction 5 hours after inoculating.
