"
Team:Valencia/Notebook/August
From 2008.igem.org
|
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
August 1st
- We carried out the experiment with Medium YPKAc. We were just characterizing yeast growth in this medium. In this medium containing acetate, yeast is supposed to only breathe rather than fermenting.
August 5th
- Experiment carried out:
SP medium.
Four strains.
No galactose activation.
August 6th
-Trying to find out the best moment for galactose activation....
SP medium.
Galactose activation at two, three and four hours.
O.D. measured at each time.
August 7th
-We added palmitic acid to see if it had any effect, especially in UCP+ strain. Experiment:
SP medium.
Strains UCP-, 76Δ and UCP+ with galactose induction and palmitic acid at 3 hours after inoculating.
Strain UCP+ just with galactose induction.
August 8th
-We prepared lab stuff and decided the following experiment we would carry out.
August 9th
-We carried out the experiment changing some variables:
SP medium.
Four strains.
Inoculation at O.D. around 0.2
Galactose induction 5 hours after inoculating.
August 11th
-The thermocouples inside the calorimeters had been always placed in the upper part, having no contact with the liquid because of contamination reasons. Our engineers team mates suggested it would be better for the thermocouples to be submerged in the liquid. We carried out an experiment to see if there are significant differences:
Four calorimeters.
Water inside.
Two submerged, two just in contact with the air inside the calorimeter.
August 12th
-We had growing problems. Apparently, our cultures were too old...
-We repeated the experiment from yesterday, changing some variables:
We incremented the shaking.
We incremented the tilt.
We obtained surprising results, our calorimeters heated the water up!
August 13th
-Still having growing problems.
-We repeated the experiment from yesterday. This time with no so much tilt.
August 14th
-We carried out an experiment both to charazterize the yeast growth and to check if the strains behave as they should, those expresing thermogenine should grow at a lower rate.
Four strains in Erlenmeyer flash inside the 30ºC shaking stove.
Galactose induction at O.D. 0.2
O.D. measurements every one and a half hours.
