Team:Valencia/Notebook/July

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<!-- {{{Año}}}/06 (Junio) -->
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{| 
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| colspan="7" style="background:#FFD062;" | <Center>[[Team:Valencia/Notebook/June|<font color="#047DB5">June</font>]]</Center>
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| [[Team:Valencia/Notebook/July#July 28th|28]]|| [[Team:Valencia/Notebook/July#July 29th|29]] || &nbsp;[[Team:Valencia/Notebook/July#July 30th|30]] || [[Team:Valencia/Notebook/July#July 31st|31]] || <Font Color="#c0c0c0">&nbsp;&nbsp;1</Font> || <Font Color="#c0c0c0">&nbsp;2</Font> || <Font Color="#c0c0c0">&nbsp;3</Font>  
| [[Team:Valencia/Notebook/July#July 28th|28]]|| [[Team:Valencia/Notebook/July#July 29th|29]] || &nbsp;[[Team:Valencia/Notebook/July#July 30th|30]] || [[Team:Valencia/Notebook/July#July 31st|31]] || <Font Color="#c0c0c0">&nbsp;&nbsp;1</Font> || <Font Color="#c0c0c0">&nbsp;2</Font> || <Font Color="#c0c0c0">&nbsp;3</Font>  
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=== July 1st ===
=== July 1st ===
-
- We prepared some glycerol stock from the four ''Saccharomyces cerevisiae'' strains we received from Eduardo Rial.
+
- We prepared some [[Team:Valencia/Notebook/Protocols#Glycerol Stock | glycerol stock]] from the four ''Saccharomyces cerevisiae'' strains we received from [http://www.cbm.uam.es/mitolab/fichapersonal.aspx?idpersona=6 Eduardo Rial]  : <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Negative control, '''UCP -'''. <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Strain containing UCP1, '''UCP +'''. <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Mutant stains over expressing UCP1, '''Gly 76Δ''' and '''Gly 175Δ'''. <br>
 +
 
- We calibrated our calorimeters with hot water.
- We calibrated our calorimeters with hot water.
=== July 2nd ===
=== July 2nd ===
-
- We prepared our first SD medium.
+
- We prepared our first [[Team:Valencia/Notebook/Protocols#SD Medium | SD medium.]]
- We calibrated our calorimeters with hot water.
- We calibrated our calorimeters with hot water.
=== July 4th ===
=== July 4th ===
-
- We prepared some plates for inoculum.
+
- We prepared some plates for inoculum. They were stored a the 28ºC stove.
=== July 7th ===
=== July 7th ===
-
- We prepared some inoculum for our experiment the following day.
+
- We prepared some inoculum in Erlenmeyer flasks for our experiment the following day. We kept them overnight in a 30ºC shaking stove.
=== July 8th ===
=== July 8th ===
-
- We carried out the pilot experiment.
+
- The experiment consisted on: <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[[Team:Valencia/Notebook/Protocols#SD Medium | SD medium]] <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Four strains <br>
 +
We left it overnight, but the air conditioning went off and the room temperature rose.
===July 9th===
===July 9th===
-
- We characterized culture growth in our calorimeters with just one strain.
+
- We characterized culture growth in our calorimeters by measuring DO, with just one strain.<br>
===July 10th===
===July 10th===
-
- Trying to see if there is basal rate of UCP1...
+
- Trying to see if there is basal rate of UCP1. Experiment: <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[[Team:Valencia/Notebook/Protocols#SD Medium | SD medium]] <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Calorimeters containing: water, medium, UCP-, Gly 175Δ.  
===July 11th===
===July 11th===
-
- It looks like our ''Saccharomyces cerevisiae'' strains have a basal rate of UCP1... We'll have to repeat the experiment to be sure.
+
- It looks like our ''Saccharomyces cerevisiae'' mutant strain has a basal rate of UCP1... We repeated the experiment to be sure.
===July 12th===
===July 12th===
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===July 15th===
===July 15th===
-
- We prepared inoculum.
+
- We prepared inoculum in Erlenmeyer flasks. We kept them overnight in a 30ºC shaking stove.
   
   
===July 16th===
===July 16th===
-
- Trying to find out which is the best moment for activating UCP1...
+
- Trying to find out which is the best moment for activating UCP1.... The glucose in the medium must be consumed by the induction time. DO at the beginning of the experiment was 0.05. <br>
-
 
+
Experiment: <br>
-
- We calculated the [[Team:Valencia/Modeling#Calculating temperature range | temperature range]] in which we expect thermogenine to work within.
+
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]] <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Galactose activation. <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; One strain. <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Activation at two, three and four hours after inoculating. D.O. was measured at each time.<br>
===July 17th===
===July 17th===
Line 158: Line 214:
===July 18th===
===July 18th===
-
- We prepared lots of plates with our SD medium...Unfortunately, we forgot to add glucose.
+
- We prepared lots of plates with our [[Team:Valencia/Notebook/Protocols#SD Medium |SD medium]]...Unfortunately, we forgot to add glucose.
-
- We carried out the pilot experiment. We see weird oscillations.
+
- We carried out the pilot experiment: <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[[Team:Valencia/Notebook/Protocols#SP liquid medium |SP medium]]. <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Galactose activation at 3 hours (approximate DO 0.15). <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Four strains. <br>
 +
We see weird oscillations.
===July 20th===
===July 20th===
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===July 21st===
===July 21st===
-
- We carried out the pilot experiment again. Weird results, we need to repeat it.
+
- We carried out the [[Team:Valencia/Notebook/July#July 18th | pilot experiment]] from July 18th again. Weird results, we need to repeat it.
===July 22nd===
===July 22nd===
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===July 23rd===
===July 23rd===
-
- We repeated the pilot experiment.
+
- We repeated the [[Team:Valencia/Notebook/July#July 18th | pilot experiment]] from July 18th.
===July 24th===
===July 24th===
-
- Our male group members decided to carried out their own particular experiment involving parafilm, plasticine and teeth filling paste...
+
- Our male group members decided to carried out their own particular experiment involving parafilm, plasticine and teeth filling paste... They wanted to find out if the oscillations would stop if they covered the thermocouples with different materials they found in the contiguuos labs, or immerse them in the liquid. <br>
 +
Meri, who is in charged of the lab, wasn't very happy the following day...
===July 25th===
===July 25th===
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===July 26th===
===July 26th===
-
- We obtain good results.
+
- One of our calorimeters wasn't working properly.
===July 28th===
===July 28th===
-
- One of our calorimeters wasn't working. We repaired it and calibrated once again.
+
- We repaired the calorimeter and calibrated once again.
===July 29th===
===July 29th===
-
- Our cultures haven't grown enough for the experiment. Temperature and medium was all right, we'll have to wait...
+
- Our cultures haven't grown enough for the experiment. Temperature and medium were all right, we'll have to wait...
===July 30th===
===July 30th===
-
- We carried out the pilot experiment. Weird results...
+
- We carried out the [[Team:Valencia/Notebook/July#July 18th | pilot experiment]], same as July 18th. Weird results...
===July 31st===
===July 31st===
-
- We carried out the pilot experiment once again.
+
- We carried out the [[Team:Valencia/Notebook/July#July 18th | pilot experiment]] once again.
 +
 
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Back to<a href="https://2008.igem.org/Team:Valencia/Notebook/June"><font color="#047DB5"> June </font></a>&nbsp;&nbsp;&nbsp;| &nbsp;&nbsp;&nbsp;July &nbsp;&nbsp;&nbsp;| &nbsp;&nbsp;&nbsp;Go to <a href="https://2008.igem.org/Team:Valencia/Notebook/August"><font color="#047DB5"> August </font></a></h2>
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Latest revision as of 20:20, 26 October 2008



April
Mo Tu We Th Fr Sa Su
31  1   2  3  4  5  6
 7  8   9 10 11 12 13
14 15  16 17 18 19 20
21 22  23 24 25 26 27
28 29  30  1  2  3  4
May
Mo Tu We Th Fr Sa Su
28 29  30  1  2  3  4
 5  6   7  8  9 10 11
12 13  14 15 16 17 18
19 20  21 22 23 24 25
26 27  28 29 30 31 1
June
Mo Tu We Th Fr Sa Su
26 27  28  29 30 31  1
 2  3   4  5  6  7  8
 9 10  11 12 13 14 15
16 17  18 19 20 21 22
23 24  25 26 27 28 29
30  1   2  3  4  5  6
July
Mo Tu We Th Fr Sa Su
30  1  2  3  4  5  6
 7  8   9 10 11 12 13
14 15  16 17 18 19 20
21 22  23 24 25 26 27
28 29  30 31   1  2  3
August
Mo Tu We Th Fr Sa Su
28 29  30 31  1  2  3
 4  5   6  7  8  9 10
11 12  13 14 15 16 17
18 19  20 21 22 23 24
25 26  27 28 29 30 31
September
Mo Tu We Th Fr Sa Su
 1  2   3  4  5  6  7
 8  9  10 11 12 13 14
15 16  17 18 19 20 21
22 23  24 25 26 27 28
29 30  1 2 3 4 5
October
Mo Tu We Th Fr Sa Su
29 30   1  2  3  4  5
 6  7   8 9 10 11 12
13 14  15 16 17 18 19
20 21  22 23 24 25 26
27 28  29 30 31  1  2
November
Mo Tu We Th Fr Sa Su
27 28  29 30 31  1  2
 3  4   5  6  7  8  9
10 11  12 13 14 15 16
17 18  19 20 21 22 23
24 25  26 27 28 29 30



July 1st

- We prepared some glycerol stock from the four Saccharomyces cerevisiae strains we received from Eduardo Rial  :
      Negative control, UCP -.
      Strain containing UCP1, UCP +.
      Mutant stains over expressing UCP1, Gly 76Δ and Gly 175Δ.


- We calibrated our calorimeters with hot water.

July 2nd

- We prepared our first SD medium.

- We calibrated our calorimeters with hot water.

July 4th

- We prepared some plates for inoculum. They were stored a the 28ºC stove.

July 7th

- We prepared some inoculum in Erlenmeyer flasks for our experiment the following day. We kept them overnight in a 30ºC shaking stove.

July 8th

- The experiment consisted on:
       SD medium
      Four strains
We left it overnight, but the air conditioning went off and the room temperature rose.

July 9th

- We characterized culture growth in our calorimeters by measuring DO, with just one strain.

July 10th

- Trying to see if there is basal rate of UCP1. Experiment:
       SD medium
      Calorimeters containing: water, medium, UCP-, Gly 175Δ.

July 11th

- It looks like our Saccharomyces cerevisiae mutant strain has a basal rate of UCP1... We repeated the experiment to be sure.

July 12th

- We obtained the same results.

July 14th

- The plate is completely dry,the water evaporeted...WHY???? the first unsolved mystery of valencia iGEM 2008.

July 15th

- We prepared inoculum in Erlenmeyer flasks. We kept them overnight in a 30ºC shaking stove.

July 16th

- Trying to find out which is the best moment for activating UCP1.... The glucose in the medium must be consumed by the induction time. DO at the beginning of the experiment was 0.05.
Experiment:
       SP medium
       Galactose activation.
       One strain.
       Activation at two, three and four hours after inoculating. D.O. was measured at each time.

July 17th

- Our lab electricity went off during the night. How many experiments will have it ruined?

July 18th

- We prepared lots of plates with our SD medium...Unfortunately, we forgot to add glucose.

- We carried out the pilot experiment:
      SP medium.
      Galactose activation at 3 hours (approximate DO 0.15).
      Four strains.
We see weird oscillations.

July 20th

- We stopped the shaking and carried out the experiment again to see whether the oscillations were related to the shaking.

July 21st

- We carried out the pilot experiment from July 18th again. Weird results, we need to repeat it.

July 22nd

- We repaired our calorimeters. They were the reason for yesterday weird results.

July 23rd

- We repeated the pilot experiment from July 18th.

July 24th

- Our male group members decided to carried out their own particular experiment involving parafilm, plasticine and teeth filling paste... They wanted to find out if the oscillations would stop if they covered the thermocouples with different materials they found in the contiguuos labs, or immerse them in the liquid.
Meri, who is in charged of the lab, wasn't very happy the following day...

July 25th

- Another curious experiment was carried out: one thermocouple was covered with plasticine while the others weren't.

July 26th

- One of our calorimeters wasn't working properly.

July 28th

- We repaired the calorimeter and calibrated once again.

July 29th

- Our cultures haven't grown enough for the experiment. Temperature and medium were all right, we'll have to wait...

July 30th

- We carried out the pilot experiment, same as July 18th. Weird results...

July 31st

- We carried out the pilot experiment once again.


Back to June    |    July    |    Go to August


Retrieved from "http://2008.igem.org/Team:Valencia/Notebook/July"