Team:Valencia/Notebook/September

From 2008.igem.org

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| &nbsp;1 || &nbsp;[[Team:Valencia/Notebook/September #September 2nd|2]] || &nbsp;&nbsp;[[Team:Valencia/Notebook/September #September 3rd|3]] || &nbsp;4 || &nbsp;[[Team:Valencia/Notebook/September #September 5th|5]] || &nbsp;6|| &nbsp;7
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| 22 || 23 || &nbsp;[[Team:Valencia/Notebook/September #September 24th|24]] || 25 || [[Team:Valencia/Notebook/September #September 26th|26]] || [[Team:Valencia/Notebook/September #September 27th|27]] || 28
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<!-- ESCRIBIR AQUI -->
<!-- ESCRIBIR AQUI -->
 +
===September 2nd ===
 +
-We carried out the following experiment: <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Four strains in LCCs with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1 % Galactose induction at the beginning. Initial O.D. 0,7 <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;275 rpm. <br>
 +
 +
-Team meeting: Checking project progress.
 +
 +
=== September 3rd ===
 +
-We characterized LCC temperature evolution with water. <br>
 +
 +
===September 5th ===
 +
-We characterized LCC temperature evolution with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]] and SP medium with bleach (to avoid contamination).
 +
 +
=== September 8th ===
 +
- We observed the culture after the experiment under the microcope. There was no contamination but we did observe some elongated microorganisms, same size as ''S. cerevisiae''. <br>
 +
 +
- We discovered that our plaque with the four strains for the following experiment had suffered cross contamination. <br>
 +
 +
=== September 9th ===
 +
-We carried out the following experiment: <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Four strains in LCCs with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1 % Galactose induction at the beginning. Initial O.D. 0,6 <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;270 rpm. <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Initial temperature 31 ºC. Too high. <br>
 +
 +
=== September 11th ===
 +
-We repeated the same experiment: <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Four strains in LCCs with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1 % Galactose induction at the beginning. Initial O.D. 0,6 <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;270 rpm. <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Initial temperature 31 ºC. Too high again. <br>
 +
 +
=== September 15th ===
 +
-We repeated the same experiment: <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Four strains in LCCs with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1 % Galactose induction at the beginning. Initial O.D. 0,6 <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;270 rpm. <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Initial temperature 28 ºC. Perfect! <br>
 +
 +
=== September 20th ===
 +
-Trying to reproduce the previous experiment...
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Four strains in LCCs with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1 % Galactose induction at the beginning. Initial O.D. 0,6 <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;270 rpm. <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Initial temperature 28 ºC. But room temperature too high <br>
 +
 +
=== September 24th ===
 +
- We repeated the same experiment: <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Four strains in LCCs with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1 % Galactose induction at the beginning. Initial O.D. 0,6 <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;270 rpm. <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Initial temperature 28 ºC.<br>
 +
 +
=== September 26th ===
 +
- Team meeting: Checking project progress and inmediate future actions.
 +
 +
=== September 27th ===
 +
- We tryied and increased initial O.D. <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Four strains in LCCs with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1 % Galactose induction at the beginning. Initial O.D. 2 <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;270 rpm. <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Initial temperature 28 ºC.<br>

Latest revision as of 16:56, 19 October 2008



April
Mo Tu We Th Fr Sa Su
31  1   2  3  4  5  6
 7  8   9 10 11 12 13
14 15  16 17 18 19 20
21 22  23 24 25 26 27
28 29  30  1  2  3  4
May
Mo Tu We Th Fr Sa Su
28 29  30  1  2  3  4
 5  6   7  8  9 10 11
12 13  14 15 16 17 18
19 20  21 22 23 24 25
26 27  28 29 30 31 1
June
Mo Tu We Th Fr Sa Su
26 27  28  29 30 31  1
 2  3   4  5  6  7  8
 9 10  11 12 13 14 15
16 17  18 19 20 21 22
23 24  25 26 27 28 29
30  1   2  3  4  5  6
July
Mo Tu We Th Fr Sa Su
30  1  2  3  4  5  6
 7  8   9 10 11 12 13
14 15  16 17 18 19 20
21 22  23 24 25 26 27
28 29  30 31   1  2  3
August
Mo Tu We Th Fr Sa Su
28 29  30 31  1  2  3
 4  5   6  7  8  9 10
11 12  13 14 15 16 17
18 19  20 21 22 23 24
25 26  27 28 29 30 31
September
Mo Tu We Th Fr Sa Su
 1  2   3  4  5  6  7
 8  9  10 11 12 13 14
15 16  17 18 19 20 21
22 23  24 25 26 27 28
29 30  1 2 3 4 5
October
Mo Tu We Th Fr Sa Su
29 30   1  2  3  4  5
 6  7   8 9 10 11 12
13 14  15 16 17 18 19
20 21  22 23 24 25 26
27 28  29 30 31  1  2
November
Mo Tu We Th Fr Sa Su
27 28  29 30 31  1  2
 3  4   5  6  7  8  9
10 11  12 13 14 15 16
17 18  19 20 21 22 23
24 25  26 27 28 29 30


September 2nd

-We carried out the following experiment:
      Four strains in LCCs with SP medium.
      1 % Galactose induction at the beginning. Initial O.D. 0,7
      275 rpm.

-Team meeting: Checking project progress.

September 3rd

-We characterized LCC temperature evolution with water.

September 5th

-We characterized LCC temperature evolution with SP medium and SP medium with bleach (to avoid contamination).

September 8th

- We observed the culture after the experiment under the microcope. There was no contamination but we did observe some elongated microorganisms, same size as S. cerevisiae.

- We discovered that our plaque with the four strains for the following experiment had suffered cross contamination.

September 9th

-We carried out the following experiment:
      Four strains in LCCs with SP medium.
      1 % Galactose induction at the beginning. Initial O.D. 0,6
      270 rpm.
       Initial temperature 31 ºC. Too high.

September 11th

-We repeated the same experiment:
      Four strains in LCCs with SP medium.
      1 % Galactose induction at the beginning. Initial O.D. 0,6
      270 rpm.
       Initial temperature 31 ºC. Too high again.

September 15th

-We repeated the same experiment:
      Four strains in LCCs with SP medium.
      1 % Galactose induction at the beginning. Initial O.D. 0,6
      270 rpm.
       Initial temperature 28 ºC. Perfect!

September 20th

-Trying to reproduce the previous experiment...       Four strains in LCCs with SP medium.
      1 % Galactose induction at the beginning. Initial O.D. 0,6
      270 rpm.
       Initial temperature 28 ºC. But room temperature too high

September 24th

- We repeated the same experiment:
      Four strains in LCCs with SP medium.
      1 % Galactose induction at the beginning. Initial O.D. 0,6
      270 rpm.
       Initial temperature 28 ºC.

September 26th

- Team meeting: Checking project progress and inmediate future actions.

September 27th

- We tryied and increased initial O.D.
      Four strains in LCCs with SP medium.
      1 % Galactose induction at the beginning. Initial O.D. 2
      270 rpm.
       Initial temperature 28 ºC.


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