Team:Warsaw/Calendar-Main/11 June 2008

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Michał L., Ewa, Marcin

Transformation of E. coli TOP10 carrying pZC320 with:

  • pMPMT5,
  • pMPMT5-AID,
  • pMPMT5-AIDT7,
  • or pMPMT5-AiD+T7.

Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).

Rifampicin test of transformants.

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