Team:Warsaw/Calendar-Main/17 October 2008

From 2008.igem.org

(Difference between revisions)
m
Line 29: Line 29:
<a name="fig2"><img src="https://static.igem.org/mediawiki/2008/e/e5/Go_17_10_2008.jpg"></a>
<a name="fig2"><img src="https://static.igem.org/mediawiki/2008/e/e5/Go_17_10_2008.jpg"></a>
-
<var><b>Fig. 2.</b>EcoRI/BcuRI digests of BBa_K103019<br>
+
<var><b>Fig. 2.</b>EcoRI/BcuRI digests of BBa_K103019.<br>
1. Marker<br>
1. Marker<br>
2. EcoRI/BcuI digest of BBa_K103019<br></var>
2. EcoRI/BcuI digest of BBa_K103019<br></var>

Revision as of 14:05, 28 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
next notebook entry

 



Preparation of OmpA-linker-omega-linker (BBa_K103016)

Michał K.

  1. Isolation of plasmids from cultures inoculated on previous day pACYC177 + OmpA-linker-omega-linker (BBa_K103016).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - no proper clones found Fig. 1.
Fig. 1.EcoRI/PstI digests of plasmids pACYC177+OmpA-linker-omega-linker (BBa_K103016).
1. Marker
2-9. EcoRI/PstI digests of pACYC177+OmpA-linker-omega-linker (BBa_K103016)

Preparation of alpha_linker under PT7 (BBa_K103019)

Michał K., Piotr

  1. Gel electrophoresis of over night digest (pSB1A3 carrying alpha_linker under PT7 (BBa_K103019)) and gel-out of proper band - 800 bp Fig. 2.
  2. Ligation of isolated DNA fragments: pSB2K3 (from 30 September) + alpha_linker under PT7 (BBa_K103019).
  3. Transformation of TOP10 with above ligation.
  4. Plating on LB with kanamycin.
Fig. 2.EcoRI/BcuRI digests of BBa_K103019.
1. Marker
2. EcoRI/BcuI digest of BBa_K103019

Preparation of AID under pBAD/araC (BBa_K103002)

Piotr

  1. Transformation of TOP10 with overnight ligation - pSB1A3 + AID under pBAD/araC (BBa_K103002).
  2. Plating on LB with ampicillin.