Team:Warsaw/Calendar-Main/24 May 2008

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<html><h3>Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7<br>
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<html><h3>Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7</h3>
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Michał K.</h3>
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<h4>Michał K.</h4>
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Revision as of 21:41, 10 October 2008

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Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7

Michał K.

  1. Isolation of hypothetical pMPM-T5 with transcription fusion from cultures.
  2. Isolation of hypothetical pMPM-T5 with translation fusion from cultures.
  3. Control digest of pMPM-T5 with transcription fusion with EcoRI (EcoRI buffer).
  4. Control digest of pMPM-T5 with translation fusion with BamHI (BamHI buffer).
  5. Gel electrophoresis (still no proper colonies found).

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