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USTC/Notebook/Preparing Antibiotic Stocks
From 2008.igem.org
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'''[[Team:USTC/Notebook|< Back to Notebook]]''' | '''[[Team:USTC/Notebook|< Back to Notebook]]''' | ||
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| + | ==Preparing Antibiotic Stocks= | ||
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| + | Ampicillin | ||
| + | |||
| + | Stocks & Usage | ||
| + | Stock Concentration - 100mg/ml in H2O | ||
| + | Aliquots - 200μl and 1ml | ||
| + | Working Concentration - 100μg/ml | ||
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| + | |||
| + | Kanamycin | ||
| + | Stocks & Usage | ||
| + | Stock Concentration - 10mg/ml in H2O | ||
| + | Aliquots - 200μl and 1ml | ||
| + | Working Concentration - 20μg/ml | ||
| + | |||
| + | Chloramphenicol | ||
| + | Stock concentration - 34mg/ml in 100% Ethanol | ||
| + | Aliquots - 1ml | ||
| + | Working concentration = 25μ/ml (Stringent), 170μ/ml (relaxed) | ||
Revision as of 13:53, 27 October 2008
=Preparing Antibiotic Stocks
Ampicillin
Stocks & Usage Stock Concentration - 100mg/ml in H2O Aliquots - 200μl and 1ml Working Concentration - 100μg/ml
Kanamycin
Stocks & Usage
Stock Concentration - 10mg/ml in H2O
Aliquots - 200μl and 1ml
Working Concentration - 20μg/ml
Chloramphenicol Stock concentration - 34mg/ml in 100% Ethanol Aliquots - 1ml Working concentration = 25μ/ml (Stringent), 170μ/ml (relaxed)
