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USTC/Notebook/Preparing Antibiotic Stocks
From 2008.igem.org
(Difference between revisions)
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'''[[Team:USTC/Notebook|< Back to Notebook]]''' | '''[[Team:USTC/Notebook|< Back to Notebook]]''' | ||
| - | + | = Preparing Antibiotic Stocks = | |
| - | =Ampicillin= | + | ===Ampicillin=== |
Stocks & Usage | Stocks & Usage | ||
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| - | =Kanamycin= | + | ===Kanamycin=== |
Stocks & Usage | Stocks & Usage | ||
Stock Concentration - 10mg/ml in H2O | Stock Concentration - 10mg/ml in H2O | ||
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Working Concentration - 20μg/ml | Working Concentration - 20μg/ml | ||
| - | =Chloramphenicol= | + | ===Chloramphenicol=== |
Stock concentration - 34mg/ml in 100% Ethanol | Stock concentration - 34mg/ml in 100% Ethanol | ||
Aliquots - 1ml | Aliquots - 1ml | ||
Working concentration = 25μ/ml (Stringent), 170μ/ml (relaxed) | Working concentration = 25μ/ml (Stringent), 170μ/ml (relaxed) | ||
Revision as of 13:54, 27 October 2008
Contents |
Preparing Antibiotic Stocks
Ampicillin
Stocks & Usage Stock Concentration - 100mg/ml in H2O Aliquots - 200μl and 1ml Working Concentration - 100μg/ml
Kanamycin
Stocks & Usage Stock Concentration - 10mg/ml in H2O Aliquots - 200μl and 1ml Working Concentration - 20μg/ml
Chloramphenicol
Stock concentration - 34mg/ml in 100% Ethanol Aliquots - 1ml Working concentration = 25μ/ml (Stringent), 170μ/ml (relaxed)
